8-OHDG in all species. No observable crossreactivity for the following analogues: guanosine (G), 7methylG, 6SHG, 8bromoG, dA, dC, dT, dI, dU, dG, O6methyldG, 8OHdA, guanine (Gua), O6methylGua, 8OHGua, uric acid, Urea, creatine, creatinine Slight but minimal (<1 %) crossreactivity for the following analogues: 8sulfhydrylG and 8OHG 1
Purification
Prepared as ascites then ammonium sulphate purified
8-OHDG
EIA, IHC (fro), IF
Hôte: Souris
Monoclonal
15A3
unconjugated
Indications d'application
Tested for IHC and ELISA. User should determine optimum titer for each application.
Restrictions
For Research Use only
Format
Lyophilized
Buffer
Lyophilized as 100 ug/mL IgG in10 mM Phosphate buffered saline, pH7.4 containing 1.0 % BSA.
Stock
-20 °C
Baila, Ohno, Nagamoto, Kotosai, Yabuuchi, Funaguchi, Ito, Endo, Mori, Takemura, Fujiwara, Fujiwara, Minatoguchi: "Tetomilast attenuates elastase-induced pulmonary emphysema through inhibition of oxidative stress in rabbits." dans: Biological & pharmaceutical bulletin, Vol. 35, Issue 4, pp. 494-502, (2012) (PubMed).
Sintara, Thong-Ngam, Patumraj, Klaikeaw: "Curcumin attenuates gastric cancer induced by N-methyl-N-nitrosourea and saturated sodium chloride in rats." dans: Journal of biomedicine & biotechnology, Vol. 2012, pp. 915380, (2012) (PubMed).
Toyokuni, Hirao, Wada, Nagai, Date, Yoshii, Akatsuka, Yamashita, Kawada: "Age- and sun exposure-dependent differences in 8-hydroxy-2'-deoxyguanosine and N-(carboxymethyl)lysine in human epidermis." dans: Journal of clinical biochemistry and nutrition, Vol. 49, Issue 2, pp. 121-4, (2011) (PubMed).
Sun, Sugiyama, Masuda, Ochi, Takeuchi: "Expressions of protein oxidation markers, dityrosine and advanced oxidation protein products in acetaminophen-induced liver injury in rats." dans: The Journal of veterinary medical science / the Japanese Society of Veterinary Science, Vol. 73, Issue 9, pp. 1185-90, (2011) (PubMed).
Calveley, Jelveh, Langan, Mahmood, Yeung, Van Dyk, Hill: "Genistein can mitigate the effect of radiation on rat lung tissue." dans: Radiation research, Vol. 173, Issue 5, pp. 602-11, (2010) (PubMed).
Rabbani, Mi, Zhang, Delong, Jackson, Fleckenstein, Salahuddin, Zhang, Clary, Anscher, Vujaskovic: "Hypoxia inducible factor 1alpha signaling in fractionated radiation-induced lung injury: role of oxidative stress and tissue hypoxia." dans: Radiation research, Vol. 173, Issue 2, pp. 165-74, (2010) (PubMed).
Mori, Nakamachi, Ohtaki, Yofu, Sato, Endo, Iso, Suzuki, Takeyama, Shintani, Hashimoto, Baba, Shioda: "Cardioprotective effect of endogenous pituitary adenylate cyclase-activating polypeptide on Doxorubicin-induced cardiomyopathy in mice." dans: Circulation journal : official journal of the Japanese Circulation Society, Vol. 74, Issue 6, pp. 1183-90, (2010) (PubMed).
Yamada, Ryo, Tai, Tamaki, Inoue, Mishima, Tsubota, Saito: "Evaluation of therapeutic effects of astaxanthin on impairments in salivary secretion." dans: Journal of clinical biochemistry and nutrition, Vol. 47, Issue 2, pp. 130-7, (2010) (PubMed).
Herbas, Ueta, Ichikawa, Chiba, Ishibashi, Shichiri, Fukumoto, Yokoyama, Takeya, Xuan, Arai, Suzuki: "Alpha-tocopherol transfer protein disruption confers resistance to malarial infection in mice." dans: Malaria journal, Vol. 9, pp. 101, (2010) (PubMed).
Raz, Zhang, Zhou, Han, Gulati, Yang, Yang, Wang, Brann: "Role of Rac1 GTPase in NADPH oxidase activation and cognitive impairment following cerebral ischemia in the rat." dans: PLoS ONE, Vol. 5, Issue 9, pp. e12606, (2010) (PubMed).
Du, Wooten, Gearing, Wooten: "Age-associated oxidative damage to the p62 promoter: implications for Alzheimer disease." dans: Free radical biology & medicine, Vol. 46, Issue 4, pp. 492-501, (2009) (PubMed).
Fujita, Miyachi, Tanaka, Takeo, Nakagawa, Kobayashi, Iwasa, Watanabe, Takei: "Iron overload is associated with hepatic oxidative damage to DNA in nonalcoholic steatohepatitis." dans: Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, Vol. 18, Issue 2, pp. 424-32, (2009) (PubMed).
Ueno, Torimura, Nakamura, Sivakumar, Nakayama, Otabe, Yuan, Yamada, Hashimoto, Inoue, Koga, Sata: "Epigallocatechin-3-gallate improves nonalcoholic steatohepatitis model mice expressing nuclear sterol regulatory element binding protein-1c in adipose tissue." dans: International journal of molecular medicine, Vol. 24, Issue 1, pp. 17-22, (2009) (PubMed).
Celino, Yamaguchi, Miura, Miura: "Arsenic inhibits in vitro spermatogenesis and induces germ cell apoptosis in Japanese eel (Anguilla japonica)." dans: Reproduction (Cambridge, England), Vol. 138, Issue 2, pp. 279-87, (2009) (PubMed).
Freeman, Parvizi, Della Valle, Steinbeck: "Reactive oxygen and nitrogen species induce protein and DNA modifications driving arthrofibrosis following total knee arthroplasty." dans: Fibrogenesis & tissue repair, Vol. 2, Issue 1, pp. 5, (2009) (PubMed).
Ribaldo, Souza, Biswas, Block, Lopes de Faria, Lopes de Faria: "Green tea (Camellia sinensis) attenuates nephropathy by downregulating Nox4 NADPH oxidase in diabetic spontaneously hypertensive rats." dans: The Journal of nutrition, Vol. 139, Issue 1, pp. 96-100, (2008) (PubMed).
Lee, Hsu, Wang, Chen, Chiu: "Chemopreventive effect of selenium and Chinese medicinal herbs on N-nitrosobis(2-oxopropyl)amine-induced hepatocellular carcinoma in Syrian hamsters." dans: Liver international : official journal of the International Association for the Study of the Liver, Vol. 28, Issue 6, pp. 841-55, (2008) (PubMed).
Umekawa, Sugiyama, Kihira, Murabayashi, Zhang, Nagao, Kamimoto, Ma, Yodoi, Sagawa: "Overexpression of thioredoxin-1 reduces oxidative stress in the placenta of transgenic mice and promotes fetal growth via glucose metabolism." dans: Endocrinology, Vol. 149, Issue 8, pp. 3980-8, (2008) (PubMed).
Chigurupati, Son, Hyun, Lathia, Mughal, Savell, Li, Nagaraju, Chan, Arumugam, Mattson: "Lifelong running reduces oxidative stress and degenerative changes in the testes of mice." dans: The Journal of endocrinology, Vol. 199, Issue 2, pp. 333-41, (2008) (PubMed).
Fujihara, Nagai, Sussan, Biswal, Handa: "Chronic cigarette smoke causes oxidative damage and apoptosis to retinal pigmented epithelial cells in mice." dans: PLoS ONE, Vol. 3, Issue 9, pp. e3119, (2008) (PubMed).
8-hydroxy-2'-deoxyguanosine (8-OHdG) is a product of oxidatively damaged DNA formed by hydroxy radical, singlet oxygen and direct photodynamic action. Anti 8-OHdG monoclonal antibody (clone N45.1) is highly specific for 8-OHdG, and is suitable for immunohistochemistry. Cross reactivity have been checked for 8-OHdG analogues (guanosine(G),7-methyl-G, 6-SH-G, 8-Bromo-G, dA, dC, dT, dI, dU, dG, O6-methyl-dG, 8-OHdA, guanine(Gua),O6-methyl-Gua, 8-OH-Gua, uric acid, urea, creatine, creatinine, 8-sulfhydryl-G, 8-OH-G).