STAT5 A/B anticorps (pTyr694)

N° du produit ABIN337157

Détail du produit anti-STAT5 A/B anticorps

Antigène: STAT5 A/B

Épitope: pTyr694

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp STAT5 A/B est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: A BLAST analysis was used to suggest cross-reactivity with Stat5 alpha from human, mouse and rat based on 100 % homology with the immunizing sequence. Cross-reactivity with Stat5 alpha from other sources has not been determined.

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide corresponding to residues surrounding Y694 of mouse STAT5 alpha protein.

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: ELISA (1:25000), IHC, IHC-P (20 - 40 μg/mL), WB (1:500 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 20-40 μg/mL. Western Blot: Expect a band approximately 91 kD in size corresponding to phosphorylated Stat5a protein in the appropriate cell lysate or extract. This phosphospecific polyclonal antibody reacts with mouse Stat5a pY694 and shows minimal reactivity by ELISA against the nonphosphorylated form of the immunizing peptide.

Commentaires:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: 4°C or -20°C, Avoid freeze-thaw cycles.

Détails sur STAT5 A/B

Antigène: STAT5 A/B

Autre désignation: STAT5A+B (STAT5 A/B Produits)

Sujet: This antibody detects: STAT5 A+B