DUSP1 anticorps (C-Term)

N° du produit ABIN337195

Détail du produit anti-DUSP1 anticorps

Antigène: DUSP1 (Dual Specificity Phosphatase 1 (DUSP1))

Épitope: AA 87-101, C-Term

Reactivité: Humain, Rat, Chien

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DUSP1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Recognizes human MKP1, Mr ~42kD. Species cross-reactivity: mouse. Predicted to cross-react with rat based on sequence homology.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Marmoset, Rat, Dog (100%) Monkey, Mouse, Panda, Bovine, Rabbit (93%) Hamster, Pig (87%) Elephant (80%).

Purification: Protein A purified

Immunogène: KLH-conjugated, synthetic peptide corresponding to amino acids 87-101 (DERSAALDGAKRDGT-C) of human MAP kinase phosphatase 1 (MKP1), with a C-terminal cysteine added for conjugation purposes. The immunizing sequence is identical in rat and mouse. Percent identity by BLAST analysis: Human, Gorilla, Marmoset, Rat, Dog (100%), Monkey, Mouse, Panda, Bovine, Rabbit (93%), Hamster, Pig (87%), Elephant (80%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (0.1 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: 0.1-1 μg/mL detects MKP1 in RIPA lysates from 3T3/A31 cells. 3T3/A31 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-MKP1 (0.3 μg/mL).

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, before the addition of glycerol to 30 %

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur DUSP1

Antigène: DUSP1 (Dual Specificity Phosphatase 1 (DUSP1))

Autre désignation: MKP-1 / DUSP1 (DUSP1 Produits)

Synonymes: anticorps CL100, anticorps HVH1, anticorps MKP-1, anticorps MKP1, anticorps PTPN10, anticorps 3CH134, anticorps Mkp1, anticorps Ptpn16, anticorps DUSP1, anticorps U19515, anticorps erp, anticorps mkp-1, anticorps cb269, anticorps sb:cb269, anticorps zgc:55920, anticorps id:ibd2586, anticorps wu:fd19g02, anticorps wu:fe50g09, anticorps XCL100, anticorps dual specificity phosphatase 1, anticorps dual specificity phosphatase 1 S homeolog, anticorps DUSP1, anticorps Dusp1, anticorps dusp1, anticorps dusp1.S

Sujet: Name/Gene ID: DUSP1
Subfamily: Dual specificity MKP
Family: Protein Phosphatase

Synonyms: DUSP1, 3CH134, Dual specificity phosphatase 1, ERK-inactivating phosphatase, HVH1, MAP kinase phosphatase 1, VH1, PTPN10, CL100, MKP-1, MKP1

ID gène: 1843

UniProt: P28562