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West Nile Virus anticorps

WNV Reactivité: West Nile Virus (WNV) ELISA, WB, IP, Neut Hôte: Souris Monoclonal 345712 unconjugated
N° du produit ABIN340476
  • Antigène Tous les produits West Nile Virus (WNV)
    West Nile Virus (WNV)
    Reactivité
    • 11
    • 1
    • 1
    West Nile Virus (WNV)
    Hôte
    • 6
    • 5
    Souris
    Clonalité
    • 6
    • 5
    Monoclonal
    Conjugué
    • 11
    Cet anticorp West Nile Virus est non-conjugé
    Application
    • 5
    • 5
    • 4
    • 4
    • 3
    • 3
    ELISA, Western Blotting (WB), Immunoprecipitation (IP), Neutralization (Neut)
    Specificité
    Recognizes West Nile Virus NS2b subunit.
    Purification
    Protein G purified
    Immunogène
    E. coli-derived rwnvNS3/NS2b.

    Type of Immunogen: Recombinant protein
    Clone
    345712
    Isotype
    IgG3
  • Indications d'application
    Approved: ELISA, IP, Neut, WB

    Usage: Western Blot, Neutralization, Immunoprecipitation, Direct ELISA. Western Blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect west nile virus NS3/NS2b complex. Using a colorimetric detection system, the detection limit for rwnvNS3/NS2b complex is approximately 5 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Neutralization of west nile virus NS3 bioactivity: This antibody has been used to inhibit/neutralize the enzymatic activity of rwnvNS3/NS2b. The antibody was preincubated with the enzyme at different molar ratios at room temperature for 30 min. The enzyme was then assayed with a peptide substrate, Ac-REKR-AFC. 50 % of the proteolytic activity was inhibited by the antibody at approximately 13 μg/mL (IC50) under conditions in which the enzyme was present at 10 μg/mL and the substrate concentration was 200uM. Considering the molecular masses of the enzyme (27kD) and the antibody (150kD), IC50 was achieved at approximately 1:4 Molar ratio of the antibody to the enzyme. Immunoprecipitation: This antibody was used at a concentration of 25 μg/mL to immunoprecipitate rwnvNS3/NS2b from conditioned cell culture medium. The recovered rwnvNS3/NS2b can be detected in Western blots using a polyclonal antibody. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect west nile virus NS3/NS2b complex. The detection limit for rwnvNS3/NS2b is approximately 1 ng/well.
    Commentaires

    Target Species of Antibody: West Nile Virus

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Reconstitution: Reconstitute, sterile TBS. If 1 mL of TBS is used, the antibody concentration will be 100 μg/mL.
    Concentration
    Lot specific
    Buffer
    Lyophilized from TBS, 5 % trehalose. Reconstitution: Reconstitute, sterile TBS. If 1 mL of TBS is used, the antibody concentration will be 100 μg/mL.
  • Antigène
    West Nile Virus (WNV)
    Autre désignation
    West Nile Virus (WNV Produits)
    Classe de substances
    Virus
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