West Nile Virus anticorps
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- Antigène Tous les produits West Nile Virus (WNV)
- West Nile Virus (WNV)
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Reactivité
- West Nile Virus (WNV)
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp West Nile Virus est non-conjugé
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Application
- ELISA, Western Blotting (WB), Immunoprecipitation (IP), Neutralization (Neut)
- Specificité
- Recognizes West Nile Virus NS2b subunit.
- Purification
- Protein G purified
- Immunogène
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E. coli-derived rwnvNS3/NS2b.
Type of Immunogen: Recombinant protein - Clone
- 345712
- Isotype
- IgG3
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- Indications d'application
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Approved: ELISA, IP, Neut, WB
Usage: Western Blot, Neutralization, Immunoprecipitation, Direct ELISA. Western Blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect west nile virus NS3/NS2b complex. Using a colorimetric detection system, the detection limit for rwnvNS3/NS2b complex is approximately 5 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Neutralization of west nile virus NS3 bioactivity: This antibody has been used to inhibit/neutralize the enzymatic activity of rwnvNS3/NS2b. The antibody was preincubated with the enzyme at different molar ratios at room temperature for 30 min. The enzyme was then assayed with a peptide substrate, Ac-REKR-AFC. 50 % of the proteolytic activity was inhibited by the antibody at approximately 13 μg/mL (IC50) under conditions in which the enzyme was present at 10 μg/mL and the substrate concentration was 200uM. Considering the molecular masses of the enzyme (27kD) and the antibody (150kD), IC50 was achieved at approximately 1:4 Molar ratio of the antibody to the enzyme. Immunoprecipitation: This antibody was used at a concentration of 25 μg/mL to immunoprecipitate rwnvNS3/NS2b from conditioned cell culture medium. The recovered rwnvNS3/NS2b can be detected in Western blots using a polyclonal antibody. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect west nile virus NS3/NS2b complex. The detection limit for rwnvNS3/NS2b is approximately 1 ng/well. - Commentaires
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Target Species of Antibody: West Nile Virus
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstitution: Reconstitute, sterile TBS. If 1 mL of TBS is used, the antibody concentration will be 100 μg/mL.
- Concentration
- Lot specific
- Buffer
- Lyophilized from TBS, 5 % trehalose. Reconstitution: Reconstitute, sterile TBS. If 1 mL of TBS is used, the antibody concentration will be 100 μg/mL.
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- Antigène
- West Nile Virus (WNV)
- Autre désignation
- West Nile Virus (WNV Produits)
- Classe de substances
- Virus
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