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CD14 anticorps

CD14 Reactivité: Souris ELISA Hôte: Souris Monoclonal biG53 unconjugated

N° du produit ABIN343180

Fiche de données

Antigène Voir toutes CD14 Anticorps

CD14
Reactivité
254

45

40

27

24

23

23

23

20

17

16

15

14

14

13

10

8

7

6

5

5

2

1

1
Souris
Hôte
226

53

25

3

1
Souris
Anticorps secondaires appropriés
Clonalité
253

54

1
Monoclonal
Conjugué
126

33

25

20

15

8

6

5

5

5

5

4

4

3

3

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Cet anticorp CD14 est non-conjugé
Application
232

89

65

57

55

52

22

16

13

12

7

6

4

4

3

3

3

3

3

2

2

2

2

2

1

1

1

1

1

1
ELISA

Specificité

Membrane bound and soluble mouse CD14. The antibody blocks LPS binding to membrane bound CD14.

Purification

Protein A affinity column

Immunogène

Monocytes of mouse

Clone

BiG53

Isotype

IgG1

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CD14 Reactivité: Humain FACS, WB, IHC, ISt, StM Hôte: Souris Monoclonal LPSR-2385 unconjugated

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CD14 Reactivité: Humain FACS, WB, IHC, ELISA Hôte: Souris Monoclonal 1D1F8 unconjugated

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CD14 Reactivité: Humain, Non-Human Primate FACS, WB, ELISA, IP Hôte: Souris Monoclonal MEM-18 unconjugated

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anti-CD14 (CD14) antibody (Biotin)
CD14 Reactivité: Humain, Non-Human Primate FACS, WB, ELISA, IP Hôte: Souris Monoclonal MEM-18 Biotin

Indications d'application

Suitable for immunostaining of CD14 positive cells and inhibition studies Binds at LPS activated mouse monocytes as well as mouse CD14 transfected cells or recombinant mouse CD14 ELISA. Inhibition of LPS binding to CD14 (Binding at LPS binding site). Up to 1 ug/ml biG53 inhibit binding of FITC-LPS (0.5ug/ml) to 6 x 105 CD14+CHO transfectants (FACS), Binding titre at mouse CD14 transfected CHO-cells: more than 20.000 (ELISA, Flow cytometry)

Restrictions

For Research Use only
Format

Lyophilized

Reconstitution

Reconstitute with bidistilled water

Concentration

1 mg/mL

Buffer

PBS, pH 7.16

Agent conservateur

Without preservative

Stock

-20 °C,-80 °C
Antigène

CD14

Autre désignation

CD14 (CD14 Produits)

Sujet

Background: biG 53 represents an excellent marker for CD14. The CD14 glycoprotein, gp 55, is present on most monocytic and macrophages like cell types: monocytes, macrophages, weekly at surface of neutrophiles like Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 Molecule has been reported to be a receptor for endotoxin. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for LPS-LBP (lipopolysaccharide binding protein)-complexes.

ID gène

12475

NCBI Accession

NP_033971

UniProt

P10810

Pathways

Signalisation TLR, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades