HSP90 alpha/beta anticorps (N-Term)

N° du produit ABIN343706

Détail du produit anti-HSP90 alpha/beta anticorps

Antigène: HSP90 alpha/beta (Heat Shock Protein 90 alpha/beta (HSP90 alpha/beta))

Épitope: N-Term

Reactivité: Humain, Souris, Boeuf (Vache)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp HSP90 alpha/beta est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificité: The antibody MBH90AB recognizes the epitope EEEVE within N-terminal part of ubiquitously expressed Hsp90 alpha and Hsp90 beta intracellular proteins with calculated Mw of 84.7 kDa and 83.3 kDa, respectively, however, migrating as 90 kDa bands under reducing SDS-PAGE conditions.

Réactivité croisée (Details): Human, Mouse, Bovine, Other not determined

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Peptide corresponding to the sequence EEVHHGEEEVEC within N-terminal part of human Hsp90.

Clone: MBH90AB

Isotype: IgG1

Information d'application

Indications d'application: Western blotting: Recommended dilution: 1 μg/mL.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Détails sur HSP90 alpha/beta

Antigène: HSP90 alpha/beta (Heat Shock Protein 90 alpha/beta (HSP90 alpha/beta))

Autre désignation: Hsp90 alpha,beta (HSP90 alpha/beta Produits)

Sujet: Hsp90 (heat shock protein 90) is one of the most abundant chaperones in the cytosol of eukaryotic cells. It interacts with various proteins, including protein kinases and transcription factors, and either facilitates their stabilization and activation or directs them for proteasomal degradation. Hsp90 thus affects multiple signaling pathways and biological processes and modulation of this single target offers the prospect of simultaneous intervence to various key points of oncogenic transformation. Hsp90 operates as a dimer in a conformational cycle driven by ATP binding and hydrolysis. There are two isoforms, alpha and beta, of vertebrate Hsp90. Whereas Hsp90 beta is expressed constitutively to a high level, Hsp90 alpha is stress-inducible and is overexpressed in many cancerous cells.,HSP90A, HSP90B, LAP-2, LAP2, EL52