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p130 anticorps

RBL2 Reactivité: Humain, Chimpanzé ELISA, WB Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN349607
  • Antigène Voir toutes p130 (RBL2) Anticorps
    p130 (RBL2) (Retinoblastoma-Like 2 (p130) (RBL2))
    Reactivité
    • 70
    • 29
    • 27
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Humain, Chimpanzé
    Hôte
    • 63
    • 8
    Lapin
    Clonalité
    • 64
    • 7
    Polyclonal
    Conjugué
    • 30
    • 6
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp p130 est non-conjugé
    Application
    • 32
    • 27
    • 14
    • 13
    • 13
    • 7
    • 7
    • 6
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    ELISA, Western Blotting (WB)
    Specificité
    This antiserum is directed against Spa310 and reacts with the Spa3120 domain of pRb2/p130 from human tissues.  Based on the sequence we expect this antibody to react as well Spa310 from chimpanzee and orangutan, and with lesser affinity, Spa310 from horse, dog, bovine, rat and opossum.
     Réactivité croisée
    Chimpanzé
    Attributs du produit
    Spa310 is a 39 aa-long polypeptide encoded by a sequence which resides in the spacer region of the tumor suppressor Rb2 gene. Rb2 is a member of the retinoblastoma (Rb) gene family. Proteins in this family, which also include pRb/p105 and pRb/p107, are important cellular factors which play well-recognized roles as tumor and growth suppressors. Both p107 and pRb2/p130 share the ability to physically interact and inhibit the kinase activity of the Cdk2/Cyclin A and Cdk2/Cyclin E complexes, which play critical roles in cell cycle regulation. Spa310 is the region of the pRb2/p130 protein that is responsible for Cdk2/Cyclin E/A inhibition. Spa310 has also been shown to suppress cell growth as observed by colony formation, and to reduce volume of tumor growth in nude mice, likely through arrest in the G 0 /G 1 phase of the cell cycle. Understandably, the Spa310 small molecule represents a potentially significant pharmaceutical product in the treatment of hyperproliferative disorders.
    Purification
    affinity purified
    Stérilité
    Sterile filtered
    Immunogène
    This affinity purified antibody was prepared by repeated immunizations with a synthetic peptide corresponding to the Spa310 sequence of pRb2/p130 protein.  A residue of cysteine was added to facilitate coupling.
    Immunogen Type: Peptide
    Isotype
    IgG
    Top Product
    Discover our top product RBL2 Anticorps primaire
  • Indications d'application
    This affinity purified antibody is suitable for ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 130 kDa in size corresponding to pRb2/p130, and a band approximately 4.2kDa in size corresponding to Spa310 peptide (latter not shown), by western blotting in the appropriate cell lysate or extract.
    Commentaires

    Gene Name: RBL2

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.39 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is three (3) months from date of opening.
    Date de péremption
    3 months
  • Antigène
    p130 (RBL2) (Retinoblastoma-Like 2 (p130) (RBL2))
    Autre désignation
    pRb2 p130 (RBL2 Produits)
    Synonymes
    anticorps RBL2, anticorps RBR2, anticorps RRB2a, anticorps PRB2, anticorps RBR-2, anticorps Rb2, anticorps p130, anticorps P130, anticorps retinoblastoma-like 2 (p130), anticorps RB transcriptional corepressor like 2, anticorps related to retinoblastoma 2, anticorps RBL2, anticorps LOC541652, anticorps Rbl2
    Sujet
    Spa310 is a 39 aa-long polypeptide encoded by a sequence which resides in the spacer region of the tumor suppressor Rb2 gene. Rb2 is a member of the retinoblastoma (Rb) gene family. Proteins in this family, which also include pRb/p105 and pRb/p107, are important cellular factors which play well-recognized roles as tumor and growth suppressors. Both p107 and pRb2/p130 share the ability to physically interact and inhibit the kinase activity of the Cdk2/Cyclin A and Cdk2/Cyclin E complexes, which play critical roles in cell cycle regulation. Spa310 is the region of the pRb2/p130 protein that is responsible for Cdk2/Cyclin E/A inhibition. Spa310 has also been shown to suppress cell growth as observed by colony formation, and to reduce volume of tumor growth in nude mice, likely through arrest in the G 0 /G 1 phase of the cell cycle. Understandably, the Spa310 small molecule represents a potentially significant pharmaceutical product in the treatment of hyperproliferative disorders.
    Synonyms: 130 kDa retinoblastoma associated protein antibody, RBR2 antibody, Retinoblastoma like 2 antibody, Retinoblastoma like protein 2 antibody, Retinoblastoma Related Gene antibody
    ID gène
    5934
    UniProt
    Q08999
    Pathways
    Cycle Cellulaire, Mitotic G1-G1/S Phases
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