ATG4C
(Autophagy related 4C Cysteine Peptidase (ATG4C))
Reactivité
Humain
Hôte
Mouton
Clonalité
Polyclonal
Conjugué
Cet anticorp ATG4C est non-conjugé
Application
Western Blotting (WB), Immunohistochemistry (IHC)
Specificité
Specific for ATG4C.
Réactivité croisée
Humain, Souris, Rat
Réactivité croisée (Details)
Other species not yet tested.
Purification
IgG
Immunogène
A synthetic peptide from human cysteine protease ATG4C (autophagin-3, APG4C, AUTL1, AUTL3) conjugated to an immunogenic carrier protein has been used as the antigen.
ATG4C
Reactivité: Humain
WB, IF, ICC
Hôte: Lapin
Polyclonal
PE
Indications d'application
IHC, WB. Use at a concentration of 10-50 μg,ml. The optimal working concentration should be determined by the end user. Not yet tested in other applications.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Reconstitute in 500 μL of sterile water. Centrifuge to remove any insoluble material.
Conseil sur la manipulation
Avoid freeze and thaw cycles.
Stock
4 °C/-20 °C
Stockage commentaire
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Date de péremption
12 months
Antigène
ATG4C
(Autophagy related 4C Cysteine Peptidase (ATG4C))
Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding the same protein, have been characterized. Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. ENZYME REGULATION: Inhibited by N-ethylmaleimide. TISSUE SPECIFICITY: Highly expressed in skeletal muscle, heart, liver and testis.,Mammalian Autophagy,ATG4C, autophagin-3, APG4C, AUTL1, AUTL3, APG4-C, FLJ14867, ATG4 autophagy related 4 homolog C (S. cerevisiae), Autophagy-related protein 4 homolog C, Autophagy-related cysteine endopeptidase 3, AUT-like 3 cysteine endopeptidase