A synthetic peptide from aa region 50-100 of extracellular domain of human STIM1 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse.
A concentration of 10-50 µg/ml is recommended. The optimal concentration should be determined by the end user. Not tested in other applications.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Conseil sur la manipulation
Avoid freeze and thaw cycles.
Stock
4 °C/-20 °C
Stockage commentaire
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Function: Plays a role in mediating Ca2+ influx following depletion of intracellular Ca2+ stores. Acts as Ca2+ sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca2+ depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca2+ release-activated Ca2+ (CRAC) channel subunit, TMEM142A/ORAI1. Subcellular location: Cell membrane, Single-pass type I membrane protein. Endoplasmic reticulum membrane, Single-pass type I membrane protein. Note: Translocates from the endoplasmic reticulum to the cell membrane in response to a depletion of intracellular calcium. Tissue specificity: Ubiquitously expressed in various human primary cells and tumor cell lines. Also known as: STIM1, GOK.