TRPA1
(Transient Receptor Potential Cation Channel, Subfamily A, Member 1 (TRPA1))
Épitope
4th Cytoplasmic Loop
Reactivité
Humain
Hôte
Mouton
Clonalité
Polyclonal
Conjugué
Cet anticorp TRPA1 est non-conjugé
Application
Western Blotting (WB), Immunohistochemistry (IHC)
Specificité
Specific for TRPA1.
Immunogène
A synthetic from the 4th cytoplasmic loop of human transient receptor potential cation channel subfamily A member 1 (TRPA1, ANKTM1) conjugated to an immunogenic carrier protein was used as the immunogen.
A concentration of 10-50 µg/ml is recommended. The optimal concentration should be determined by the end user.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Conseil sur la manipulation
Avoid freeze and thaw cycles.
Stock
4 °C/-20 °C
Stockage commentaire
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Date de péremption
12 months
Antigène
TRPA1
(Transient Receptor Potential Cation Channel, Subfamily A, Member 1 (TRPA1))
The structure of the protein encoded by this gene is highly related to both the protein ankyrin andtransmembrane proteins. The specific function of this protein has not yet been determined, however, studies indicate the function may involve a role in signal transduction and growth control. Function: Receptor-activated non-selective cation channel involved in detection of sensations such as cold, pain or sounds perception. Has a central role in the pain response to endogenous inflammatory mediators and to a diverse array of volatile irritants, such as mustard oil, garlic and acrolein, an irritant from tears gas and vehicule exhaust fumes. Also involved in pain sensation triggered by capsaicin, the pungent ingredient in chilli peppers. Acts also as a ionotropic cannabinoid receptor by being activated by delta(9)-tetrahydrocannabinol (THC), the psychoactive component of marijuana. Not involved in menthol sensation. May be a component for the mechanosensitive transduction channel of hair cells in inner ear, thereby participating in the perception of sounds. Probably operated by a phosphatidylinositol second messenger system. Subcellular location: Membrane, Multi-pass membrane protein (Probable). Tissue specificity: Expressed at very low level. Expressed at very low level in human fibroblasts and at a moderate level in liposarcoma cells. Miscellaneous: Inhibited by ruthenium red, a potent blocker of TRPV channels. Also known as: Transient receptor potential cation channel subfamily A member 1, ankyrin-like withtransmembrane domains protein 1, transformation sensitive protein p120, ANKTM1.