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MBD3 anticorps (C-Term)

MBD3 Reactivité: Humain, Souris WB, IHC (p), EIA Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN356576
  • Antigène Voir toutes MBD3 Anticorps
    MBD3 (Methyl-CpG Binding Domain Protein 3 (MBD3))
    Épitope
    • 14
    • 10
    • 7
    • 6
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivité
    • 69
    • 28
    • 16
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Humain, Souris
    Hôte
    • 58
    • 9
    • 2
    Lapin
    Clonalité
    • 55
    • 14
    Polyclonal
    Conjugué
    • 41
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    Cet anticorp MBD3 est non-conjugé
    Application
    • 45
    • 29
    • 9
    • 6
    • 5
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
    Specificité
    This antibody is specific to MBD3 (C-term).
    Purification
    Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
    Immunogène
    This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding E279 of human MBD3.
    Isotype
    Ig Fraction
    Top Product
    Discover our top product MBD3 Anticorps primaire
  • Indications d'application
    ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.25 mg/mL
    Buffer
    PBS containing 0.09 % (W/V) Sodium Azide as preservative.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Avoid repeated freezing and thawing.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
  • Antigène
    MBD3 (Methyl-CpG Binding Domain Protein 3 (MBD3))
    Autre désignation
    MBD3 (MBD3 Produits)
    Synonymes
    anticorps xmbd3, anticorps MGC69548, anticorps MBD3, anticorps DKFZp459N1635, anticorps AI181826, anticorps AU019209, anticorps methyl-CpG binding domain protein 3 S homeolog, anticorps methyl-CpG binding domain protein 3, anticorps mbd3.S, anticorps mbd3, anticorps MBD3, anticorps Mbd3
    Sujet
    DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD) (1). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA (2). MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases (3). In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract (4).Synonyms: Methyl-CpG-binding domain protein 3
    ID gène
    53615, 9606
    UniProt
    O95983
    Pathways
    Chromatin Binding
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