UBA2 anticorps (C-Term)

N° du produit ABIN356597

Détail du produit anti-UBA2 anticorps

Antigène: UBA2 (SUMO1 Activating Enzyme Subunit 2 (UBA2))

Épitope: C-Term

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp UBA2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificité: This antibody is specific to UBA2 (C-term).

Purification: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogène: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human UBA2.

Isotype: Ig Fraction

Information d'application

Indications d'application: ELISA: 1/1,000. Western blot: 1/100approx. 1/500. Immunohistochemistry: 1/50approx. 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS containing 0.09 % (W/V) Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Détails sur UBA2

Antigène: UBA2 (SUMO1 Activating Enzyme Subunit 2 (UBA2))

Autre désignation: SAE2 / UBA2 (UBA2 Produits)

Synonymes: anticorps ATSAE2, anticorps EMB2764, anticorps EMBRYO DEFECTIVE 2764, anticorps F3K23.23, anticorps F3K23_23, anticorps SUMO-ACTIVATING ENZYME 2, anticorps SUMO-activating enzyme 2, anticorps ARX, anticorps SAE2, anticorps AA986091, anticorps Arx, anticorps Sae2, anticorps UBA1, anticorps Ubl1a2, anticorps Uble1b, anticorps sae2, anticorps sae2-B, anticorps uba2-a, anticorps uba2-b, anticorps uble1b-B, anticorps sae2b, anticorps uble1b, anticorps wu:fi17g06, anticorps zgc:66354, anticorps SUMO1 activating enzyme subunit 2, anticorps SUMO-activating enzyme 2, anticorps ubiquitin like modifier activating enzyme 2, anticorps ubiquitin-like modifier activating enzyme 2, anticorps ubiquitin like modifier activating enzyme 2 L homeolog, anticorps MICPUN_63834, anticorps MICPUCDRAFT_36729, anticorps SAE2, anticorps UBA2, anticorps Uba2, anticorps uba2.L, anticorps uba2

Sujet: Ubiquitin is covalently attached to target proteins by a multienzymatic system consisting of E1 (ubiquitin-activating), E2 (ubiquitin-conjugating), and E3 (ubiquitin-ligating) enzymes. NEDD8, a ubiquitin-like protein, is conjugated to proteins in a manner analogous to ubiquitinylation. beta-amyloid precursor protein-binding protein-1 (APPBP1) can bind to NEDD8 in rabbit reticulocyte lysates. However, since APPBP1 shows similarity to only the N-terminal domain of an E1 enzyme, it must interact with a protein showing similarity to the C-terminal region of E1s. By searching sequence databases, a cDNAs encoding UBA3 was identified as the human homolog of yeast Uba3. The predicted 442-amino acid UBA3 protein shares 43 % sequence identity with yeast Uba3. In vitro, UBA3 formed a complex with APPBP1 and a thioester linkage with NEDD8. APPBP1/UBA3 complex may function as an E1-like enzyme for the activation of NEDD8.Synonyms: ARX, Anthracycline-associated resistance ARX, HRIHFB2115, SUMO-activating enzyme subunit 2, UBLE1B, Ubiquitin-like 1-activating enzyme E1B

ID gène: 10054, 9606

UniProt: Q9UBT2