Protein A column, followed by peptide affinity purification. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogène
KLH conjugated synthetic peptide selected from the N-terminal region of human ALDH6A1
ELISA 1: 1,000. Western blot 1: 50 - 1: 100. Immunohistochemistry 1: 10 - 1: 50. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Concentration
0.25 mg/mL
Buffer
PBS with 0.09 % (W/V) sodium azide
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
4 °C/-20 °C
Stockage commentaire
Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.
Antigène
ALDH6A1
(Aldehyde Dehydrogenase 6 Family, Member A1 (ALDH6A1))
anticorps MMSADHA, anticorps MMSDH, anticorps Mmsdh, anticorps cb850, anticorps wu:fb03a04, anticorps zgc:92082, anticorps 1110038I05Rik, anticorps AI314632, anticorps aldehyde dehydrogenase 6 family member A1, anticorps aldehyde dehydrogenase 6 family, member A1, anticorps aldehyde dehydrogenase 6 family member A1 L homeolog, anticorps methylmalonic acid semialdehyde dehydrogenase, anticorps methylmalonate-semialdehyde dehydrogenase (CoA acylating), anticorps aldehyde dehydrogenase family 6, subfamily A1, anticorps ALDH6A1, anticorps Aldh6a1, anticorps aldh6a1.L, anticorps CCNA_02357, anticorps aldh6a1, anticorps VAA_RS07845
Sujet
ALDH6A1 belongs to the aldehyde dehydrogenases family of proteins. This enzyme plays a role in the valine and pyrimidine catabolic pathways. This protein is a mitochondrial methylmalonate semialdehyde dehydrogenase, and catalyzes the irreversible oxidative decarboxylation of malonate and methylmalonate semialdehydes to acetyl- and propionyl-CoA. Methylmalonate semialdehyde dehydrogenase deficiency is characterized by elevated beta-alanine, 3-hydroxypropionic acid, and both isomers of 3-amino and 3-hydroxyisobutyric acids in urine organic acids.Synonyms: Aldehyde dehydrogenase family 6 member A1, Malonate-semialdehyde dehydrogenase [acylating], Methylmalonate-semialdehyde dehydrogenase [acylating] mitochondrial