Separase anticorps (pSer1126)

N° du produit ABIN358223

Détail du produit anti-Separase anticorps

Antigène: Separase (ESPL1) (Extra Spindle Poles Like 1 (ESPL1))

Épitope: pSer1126

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Separase est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificité: This antibody detects SEPARIN pSer1126.

Purification: Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogène: This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S1126 of human SEPARIN.

Isotype: Ig Fraction

Information d'application

Indications d'application: ELISA: 1/1,000. Dot Blot: 1/500. Immunohistochemistry: 1/50approx. 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Détails sur Separase

Antigène: Separase (ESPL1) (Extra Spindle Poles Like 1 (ESPL1))

Autre désignation: Separin / ESPL1 (ESPL1 Produits)

Synonymes: anticorps ESP1, anticorps SEPA, anticorps mp:zf637-3-001691, anticorps AL024103, anticorps AU045071, anticorps Cerp, anticorps PRCE, anticorps SSE, anticorps extra spindle pole bodies like 1, separase, anticorps extra spindle poles like 1, anticorps extra spindle pole bodies 1, separase, anticorps ESPL1, anticorps espl1, anticorps Espl1

Sujet: The metaphase-to-anaphase transition is the final discrete event in duplication and separation of the genetic material of the cell. Its timing is regulated by the activation of the anaphase-promoting complex (APC). In both budding and fission yeast, the degradation of the Pds1 or Cut2 protein, respectively, is required for the onset of sister chromatid separation. Both proteins are APC substrates. Pds1 and Cut2 proteins associate with the yeast separin proteins Esp1 and Cut1, respectively, and prevent the separins from promoting chromatid separation. Pds1 and Cut2 are also called anaphase inhibitors or securinsSynonyms: Caspase-like protein ESPL1, ESP1, Extra spindle poles-like 1 protein, KIAA0165, Separase

Poids moléculaire: 233113 Da

ID gène: 9700, 9606

UniProt: Q14674