This antibody detects PI3 Kinase p85 (PIK3R3) at C-term.
Purification
Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogène
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 324~353 amino acids from the C-terminal region of Human PI3KR3. Genename: PIK3R3
PIK3R3
Reactivité: Humain
WB
Hôte: Lapin
Monoclonal
unconjugated
Indications d'application
ELISA: 1/1,000. Western blotting: 1/100-1/500. Immunohistochemistry: 1/10-1/50. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Concentration
0.25 mg/mL
Buffer
PBS with 0.09 % (W/V) Sodium Azide as preservative.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
4 °C/-20 °C
Stockage commentaire
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
PI3KR3 binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1. It is a component of a heterodimer of p110 (catalytic) and p55 (regulatory) subunits. The protein is expressed at highest levels in brain and testis. Lower levels are detected in adipose tissue, kidney, heart, lung and skeletal muscle. The protein contains 2 SH2 domains.Synonyms: PI3K regulatory subunit gamma, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit gamma, Phosphatidylinositol 3-kinase regulatory subunit gamma, p55PIK