PIP5K1A anticorps (N-Term)
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- Antigène Voir toutes PIP5K1A Anticorps
- PIP5K1A (Phosphatidylinositol-4-Phosphate 5-Kinase, Type I, alpha (PIP5K1A))
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Épitope
- N-Term
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Reactivité
- Humain
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp PIP5K1A est non-conjugé
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Application
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- Specificité
- This antibody reacts to PIP5K1A.
- Purification
- Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
- Immunogène
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human PIP5K1A.
- Isotype
- Ig Fraction
- Top Product
- Discover our top product PIP5K1A Anticorps primaire
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- Indications d'application
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ELISA: 1/1,000. Western blotting: 1/100 - 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- PBS with 0.09 % (W/V) sodium azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid repeated freezing and thawing.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- Antigène
- PIP5K1A (Phosphatidylinositol-4-Phosphate 5-Kinase, Type I, alpha (PIP5K1A))
- Autre désignation
- PIP5K1A (PIP5K1A Produits)
- Synonymes
- anticorps Pip5k1b, anticorps pip5k1c, anticorps PIP5K1A, anticorps pip5k1b, anticorps Pipk5a, anticorps Pipk5b, anticorps pip5k1a, anticorps zgc:110576, anticorps im:7140933, anticorps zgc:158838, anticorps phosphatidylinositol-4-phosphate 5-kinase type 1 alpha, anticorps phosphatidylinositol-4-phosphate 5-kinase, type 1, alpha, anticorps phosphatidylinositol-4-phosphate 5-kinase, type I, alpha L homeolog, anticorps phosphatidylinositol-4-phosphate 5-kinase, type I, alpha, anticorps phosphatidylinositol-4-phosphate 5-kinase, type 1 alpha, anticorps phosphatidylinositol-4-phosphate 5-kinase, type I, alpha, a, anticorps phosphatidylinositol-4-phosphate 5-kinase, type I, alpha, b, anticorps PIP5K1A, anticorps Pip5k1a, anticorps pip5k1a.L, anticorps pip5k1a, anticorps pip5k1aa, anticorps pip5k1ab
- Sujet
- Overexpression of phosphatidylinositol phosphate 5-kinase alpha (PIP5KIalpha), which synthesizes PIP2, suppresses apoptosis, whereas a kinase-deficient mutant does not. Protection by the wild-type PIP5KIalpha isaccompanied by decreases in the generation of activated caspases and of caspase 3-cleaved PARP. Protection is not mediated through PIP3 or Akt activation. An anti-apoptotic role for PIP(2) is substantiated by the finding that PIP5KIalpha is cleaved by caspase 3 during apoptosis, and cleavage inactivates PIP5KIalpha in vitro. Mutation of the P(4) position (D279A) of the PIP5KIalpha caspase 3 cleavage consensus prevents cleavage in vitro, and during apoptosis in vivo. Significantly, the caspase 3-resistant PIP5KIalpha mutant is more effective in suppressing apoptosis than the wild-type kinase. PIP2 is a direct regulator of apical and effector caspases in the death receptor and mitochondrial pathways, and PIP5KIalpha inactivation contributes to the progression of apoptosis.Synonyms: 68 kDa type I phosphatidylinositol-4-phosphate 5-kinase alpha, PIP5K1-alpha, PIP5KIalpha, Phosphatidylinositol-4-phosphate 5-kinase type-1 alpha, PtdIns(4)P-5-kinase 1 alpha
- ID gène
- 8394, 9606
- UniProt
- Q99755
- Pathways
- Signalisation PI3K-Akt, Mitotic G1-G1/S Phases, Inositol Metabolic Process, DNA Replication, Cell-Cell Junction Organization, Synthesis of DNA
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