ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Concentration
0.25 mg/mL
Buffer
PBS with 0.09 % (W/V) sodium azide
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
4 °C/-20 °C
Stockage commentaire
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Antigène
PRKAR1A
(Protein Kinase, CAMP-Dependent, Regulatory, Type I, alpha (Tissue Specific Extinguisher 1) (PRKAR1A))
anticorps ACRDYS1, anticorps ADOHR, anticorps CAR, anticorps CNC, anticorps CNC1, anticorps PKR1, anticorps PPNAD1, anticorps PRKAR1, anticorps TSE1, anticorps 1300018C22Rik, anticorps RIalpha, anticorps Tse-1, anticorps Tse1, anticorps RIIA, anticorps prkar1a, anticorps zgc:92515, anticorps im:7047729, anticorps cnc1, anticorps pkr1, anticorps ppnad1, anticorps prkar1, anticorps tse1, anticorps protein kinase cAMP-dependent type I regulatory subunit alpha, anticorps protein kinase, cAMP dependent regulatory, type I, alpha, anticorps protein kinase cAMP-dependent type 1 regulatory subunit alpha, anticorps protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1) a, anticorps cAMP-dependent protein kinase type I-alpha regulatory subunit pseudogene, anticorps protein kinase cAMP-dependent type I regulatory subunit alpha S homeolog, anticorps PRKAR1A, anticorps Prkar1a, anticorps prkar1aa, anticorps LOC707275, anticorps prkar1a.S
Sujet
CAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase (AMPK), which transduces the signal through phosphorylation of different target proteins. The inactive holoenzyme of AMPK is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits of AMPK have been identified in humans. PKR1 is one of the regulatory subunits. This protein was found to be a tissue-specific extinguisher that down-regulates the expression of seven liver genes in hepatoma x fibroblast hybrids. Functional null mutations in the gene cause Carney complex (CNC), an autosomal dominant multiple neoplasia syndrome. The gene can fuse to the RET protooncogene by gene rearrangement and form the thyroid tumor-specific chimeric oncogene known as PTC2.Synonyms: CAR, CNC1, PKR1, PRKAR1, TSE1, Tissue-specific extinguisher 1, cAMP-dependent protein kinase type I-alpha regulatory subunit