INA anticorps

N° du produit ABIN361343

Détail du produit anti-INA anticorps

Antigène: INA (Internexin Neuronal Intermediate Filament Protein, alpha (INA))

Reactivité: Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp INA est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Specificité: Specific for the ~66k alpha Internexin protein. Can be used on formalin-fixed cells in tissue culture, cryostat sections, and Western blotting. The epitope recognized by the ID2 clone is in the C-terminal non-helical extension of the protein and is unusually resistant to aldehyde fixation, so that this antibody is ideal for studies of paraffin embedded formalin fixed histological sections.

Réactivité croisée: Humain, Souris, Rat (Rattus)

Homologie: most mammals

Purification: Protein G purified culture supernatant

Immunogène: Recombinant rat alpha-internexin expressed in and purified from E. coli

Clone: 1D2

Isotype: IgG1

Information d'application

Indications d'application: Recommended Dilution: WB: 1:2,000 IF: 1:250 IHC: 1:1,000 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: total IgG fraction + 10 mM Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Détails sur INA

Antigène: INA (Internexin Neuronal Intermediate Filament Protein, alpha (INA))

Autre désignation: INA (INA Produits)

Synonymes: anticorps NEF5, anticorps NF-66, anticorps TXBP-1, anticorps Inexa, anticorps Intlaa, anticorps Nf66, anticorps AV028420, anticorps NF66, anticorps ina-A, anticorps xefiltin, anticorps ina-a, anticorps inexa, anticorps MGC82496, anticorps INA, anticorps si:ch211-67n3.6, anticorps nef5, anticorps xina, anticorps nf-66, anticorps txbp-1, anticorps MGC69277, anticorps gefiltin, anticorps zgc:110516, anticorps internexin neuronal intermediate filament protein alpha, anticorps internexin neuronal intermediate filament protein, alpha, anticorps internexin neuronal intermediate filament protein alpha S homeolog, anticorps low molecular weight neuronal intermediate filament S homeolog, anticorps internexin neuronal intermediate filament protein, alpha a, anticorps internexin neuronal intermediate filament protein, alpha b, anticorps INA, anticorps Ina, anticorps ina.S, anticorps nif.S, anticorps inaa, anticorps ina, anticorps inab

Sujet: Alpha-internexin is a Class IV intermediate filament originally discovered as it co-purifies with other neurofilament subunits (1). Alpha-internexin is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. It is expressed only in neurons and in large amounts early in neuronal development, but is down-regulated in many neurons as development proceeds. Many classes of mature neurons contain alpha-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons alpha-internexin is the only neurofilament subunit expressed. Antibodies to alpha-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition, recent studies show a marked up-regulation of alpha-internexin during neuronal regeneration (2). The use of antibodies to this protein in the study of brain tumors has not been examined to date, but is likely to be of interest. Recently Cairns et al. used this antibody to show that alpha-internexin is an abundant component of the inclusions of neurofilament inclusion body disease (NFID), a serious human neurodegenerative disorder (3,4). The antibody was also used to confirm the presence of circulating auto-antibodies to alpha-internexin in the sera of some patients with endocrine autoimmunity, as well as in some normal individuals (5). Anti-alpha Internexin Western blot of rat cortex lysate showing specific immunolabeling of the ~ 66k alpha internexin protein.

Poids moléculaire: '66 kDa

ID gène: 24503

UniProt: P23565