MAP2 anticorps

N° du produit ABIN361345

Détail du produit anti-MAP2 anticorps

Antigène: MAP2 (Microtubule-Associated Protein 2 (MAP2))

Reactivité: Boeuf (Vache)

Hôte: Poulet

Clonalité: Polyclonal

Conjugué: Cet anticorp MAP2 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificité: Specific for the ~ 280k MAP2 protein.

Réactivité croisée: Boeuf (Vache), Humain, Souris, Rat (Rattus)

Sensibilité: The antibody has been directly tested for reactivity in bovine, human, mouse and rat. It is expected that the antibody will react with other mammalian tissues.

Purification: Total IgY fraction

Immunogène: recombinant bovine MAP2 protein expressed in and purified from E. Coli

Information d'application

Indications d'application: Recommended Dilution: WB: 1:20,000 IF: 1:2,500 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: total IgY fraction in PBS + 10 mM Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Références pour anticorps anti-MAP2 (ABIN361345)

Woike, Wang, Tibbe, Hassani Nia, Failla, Kibæk, Overgård, Larsen, Fagerberg, Barsukov, Kreienkamp: "Mutations affecting the N-terminal domains of SHANK3 point to different pathomechanisms in neurodevelopmental disorders." dans: Scientific reports, Vol. 12, Issue 1, pp. 902, (2022) (PubMed).

Hassani Nia, Woike, Bento, Niebling, Tibbe, Schulz, Hirnet, Skiba, Hönck, Veith, Günther, Scholz, Bierhals, Driemeyer, Bend, Failla, Lohr, Alai, Kreienkamp: "Structural deficits in key domains of Shank2 lead to alterations in postsynaptic nanoclusters and to a neurodevelopmental disorder in humans." dans: Molecular psychiatry, (2022) (PubMed).

Hassani Nia, Woike, Martens, Klüssendorf, Hönck, Harder, Kreienkamp: "Targeting of δ-catenin to postsynaptic sites through interaction with the Shank3 N-terminus." dans: Molecular autism, Vol. 11, Issue 1, pp. 85, (2021) (PubMed).

Hassani Nia, Woike, Kloth, Kortüm, Kreienkamp: "Truncating mutations in SHANK3 associated with global developmental delay interfere with nuclear β-catenin signaling." dans: Journal of neurochemistry, (2020) (PubMed).

Ho, Dallalzadeh, Karathanasis, Keles, Vangala, Grogan, Poirazi, Martin: "GluA2 mRNA distribution and regulation by miR-124 in hippocampal neurons." dans: Molecular and cellular neurosciences, Vol. 61, pp. 1-12, (2014) (PubMed).

Détails sur MAP2

Antigène: MAP2 (Microtubule-Associated Protein 2 (MAP2))

Autre désignation: MAP2 (MAP2 Produits)

Synonymes: anticorps MAP2A, anticorps MAP2B, anticorps MAP2C, anticorps MAP2R, anticorps Mtap2, anticorps MAP-2, anticorps Mtap-2, anticorps xmap2, anticorps MGC53131, anticorps a730034c02, anticorps zgc:103474, anticorps zgc:113974, anticorps microtubule associated protein 2, anticorps microtubule-associated protein 2, anticorps microtubule associated protein 2 S homeolog, anticorps MAP2, anticorps Map2, anticorps map2.S, anticorps map2

Sujet: Microtubules are 25nm diameter protein rods found in most kinds of eukaryotic cells. They are polymerized from a dimeric subunit made of one a subunit and one b tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein ( (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5 (Kindler & Gardner 1994). MAP2 is made up of two ~280kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70kDa on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein ( (tau). MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. MAP2 has been recently shown to be the specific receptor for the neurosteroid pregnenolone (Fontaine-Lenore V. et al., 2006). Anti-MAP2 Left: Western blot of rat cortex lysate showing specific immunolabeling of the ~280k MAP2 protein. Right:Mixed neuron/glial cultures. The perikarya and dendrites of neurons are strongly and specifically stained with the MAP2 antibody (red). Cell nuclei are visualized with DAPI DNA stain.

Poids moléculaire: '280 kDa

ID gène: 4133

UniProt: P11137