Fibrillarin anticorps

N° du produit ABIN361375

Détail du produit anti-Fibrillarin anticorps

Antigène: Fibrillarin (FBL)

Reactivité: Saccharomyces cerevisiae

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Fibrillarin est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Specificité: Specific for the ~34kDa Fibrillarin /Nop1p protein. It is expected that it will work on other mammal tissues.

Réactivité croisée: Humain, Souris, Rat (Rattus)

Purification: Protein G purified culture supernatant

Immunogène: Yeast nuclear preparations

Clone: 38F3

Isotype: IgG1

Information d'application

Indications d'application: Recommended Dilution: WB: 1:1,000 IF: 1:500 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: total IgG fraction + 10 mM Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Références pour anticorps anti-Fibrillarin (ABIN361375)

Vendramin, Verheyden, Ishikawa, Goedert, Nicolas, Saraf, Armaos, Delli Ponti, Izumikawa, Mestdagh, Lafontaine, Tartaglia, Takahashi, Marine, Leucci: "SAMMSON fosters cancer cell fitness by concertedly enhancing mitochondrial and cytosolic translation." dans: Nature structural & molecular biology, Vol. 25, Issue 11, pp. 1035-1046, (2019) (PubMed).

Langhendries, Nicolas, Doumont, Goldman, Lafontaine: "The human box C/D snoRNAs U3 and U8 are required for pre-rRNA processing and tumorigenesis." dans: Oncotarget, Vol. 7, Issue 37, pp. 59519-59534, (2018) (PubMed).

Détails sur Fibrillarin

Antigène: Fibrillarin (FBL)

Autre désignation: Fibrillarin (Nop1p) (FBL Produits)

Synonymes: anticorps FIB, anticorps FLRN, anticorps RNU3IP1, anticorps wu:fb37g12, anticorps zgc:56145, anticorps zgc:77130, anticorps FBL, anticorps AL022665, anticorps CG9888, anticorps Dmel\\CG9888, anticorps Fibri, anticorps GCR-6, anticorps GCR6, anticorps Pen59C5, anticorps fib, anticorps pen59C5, anticorps MGC76139, anticorps fbl, anticorps fibrillarin, anticorps Fibrillarin, anticorps putative fibrillarin, anticorps fibrillarin S homeolog, anticorps FBL, anticorps fbl, anticorps Fbl, anticorps Fib, anticorps fib1, anticorps LMJF_19_0100, anticorps fbl.S

Sujet: Nop1p was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae. The Nop1p protein is 327 amino acids in size (34.5kDa), is essential for yeast viability, and is localized in the nucleoli (1). The systematic name for S. cerevisiae Nop1 is YDL014W, and it is now known to be part of the small subunit processome complex, involved in the processing of pre-18S ribosomal RNA. Nop1p is the yeast homologue of a protein found in all eukaryotes and archea generally called fibrillarin (2). Fibrillarin/Nop1p is extraordinarily conserved, so that the yeast and human proteins are 67% identical, and the human protein can functionally replace the yeast protein. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a ~34kDa protein which was subsequently found to be fibrillarin. Recent studies show that knock-out of the fibrillarin gene in mice results in embryonic lethality, although mice with only one functional fibrillarin/Nop1p gene were viable (3). This antibody is becoming widely used as a convenient marker for nucleoli in a wide variety of species (e.g. 4-6).

Poids moléculaire: '34 kDa

ID gène: 851548

UniProt: P15646

Pathways: Ribonucleoside Biosynthetic Process