Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.
Restrictions
For Research Use only
Format
Liquid
Buffer
100 μL in 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.
Stock
-20 °C
Smith, Jung, Jeon, Kim: "Arid1b haploinsufficiency in parvalbumin- or somatostatin-expressing interneurons leads to distinct ASD-like and ID-like behavior." dans: Scientific reports, Vol. 10, Issue 1, pp. 7834, (2020) (PubMed).
Milinkeviciute, Henningfield, Muniak, Chokr, Green, Cramer: "Microglia Regulate Pruning of Specialized Synapses in the Auditory Brainstem." dans: Frontiers in neural circuits, Vol. 13, pp. 55, (2020) (PubMed).
Santos, Fuertes, Short, Donohue, Shao, Quintanilla, Malakzadeh, Cohen-Cory: "DSCAM differentially modulates pre- and postsynaptic structural and functional central connectivity during visual system wiring." dans: Neural development, Vol. 13, Issue 1, pp. 22, (2018) (PubMed).
Rotschafer, Cramer: "Developmental Emergence of Phenotypes in the Auditory Brainstem Nuclei of Fmr1 Knockout Mice." dans: eNeuro, Vol. 4, Issue 6, (2018) (PubMed).
Rotschafer, Marshak, Cramer: "Deletion of Fmr1 alters function and synaptic inputs in the auditory brainstem." dans: PLoS ONE, Vol. 10, Issue 2, pp. e0117266, (2015) (PubMed).
Korn, Koppel, Li, Mehta, Mehta, Seidl, Cramer: "Astrocyte-secreted factors modulate the developmental distribution of inhibitory synapses in nucleus laminaris of the avian auditory brainstem." dans: The Journal of comparative neurology, (2011) (PubMed).
Antigène
SLC32A1
(Solute Carrier Family 32 (GABA Vesicular Transporter), Member 1 (SLC32A1))
The Vesicular GABA Amino Acid Transporter (VGAT) is responsible for transport of the inhibitory neurotransmitter into synaptic vesicles(McIntire et al., 1997). The VGAT protein (also known as the Vesicular Inhibitory Amino Aid Transporter or VIAAT) is expressed in synaptic vesicles of both glycine and GABAergic synapses throughout the CNS (Chaudhry et al., 1998). Expression of the VGAT protein changes during development and also in response to patterns of neuronal activity (De et al., 2005). Anti-Vesicular GABA Transporter (VGAT) Western blot of rat hippocampal lysate showing specific immunolabeling of the ~53k VGAT protein.