GLRA1 anticorps (N-Term)

N° du produit ABIN361432

Détail du produit anti-GLRA1 anticorps

Antigène: GLRA1 (Glycine Receptor, alpha 1 (GLRA1))

Épitope: N-Term

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GLRA1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Specificité: Specific for the ~48k a1- and a2-subunits of the glycine receptor in Western blots of rat spinal cord and brain stem and in cell extracts. Immunolabeling blocked by preadsorption of antibody with the peptide immunogen. Does not recognize other glycine receptor subunits.

Réactivité croisée: Humain, Souris, Rat (Rattus)

Purification: Antigen Affinity Purified from Pooled Serum

Immunogène: Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH

Information d'application

Indications d'application: Recommended Dilution: WB: 1:1000 IHC (frozen sections, unpublished observations): 1:1000 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: lyophilized

Stock: -20 °C

Détails sur GLRA1

Antigène: GLRA1 (Glycine Receptor, alpha 1 (GLRA1))

Autre désignation: GLRA1 (GLRA1 Produits)

Synonymes: anticorps GLYRA1, anticorps HKPX1, anticorps STHE, anticorps [a]Z1, anticorps B230397M16Rik, anticorps nmf11, anticorps oscillator, anticorps ot, anticorps spasmodic, anticorps spd, anticorps glycine receptor alpha 1, anticorps glycine receptor, alpha 1, anticorps glycine receptor, alpha 1 subunit, anticorps GLRA1, anticorps Glra1, anticorps glra1

Sujet: Glycine is an important inhibitory transmitter in the brainstem and spinal cord. Glycine receptors are members of the ligand-gated ion channel family (LGICs) that mediate rapid chemical neurotransmission (Schofield et al., 2003). The binding of glycine to its receptor produces a large increase in chloride conductance, which causes membrane hyperpolarization. Glycine receptors are anchored at inhibitory chemical synapses by a cytoplasmic protein, gephyrin (Fischer et al., 2000). The glycine receptor has been used to great advantage in the identification of the binding sites for alcohol on the LGIC family of proteins (Beckstead et al., 2001, Mihic et al., 1997). These receptors have also been extremely useful in studies of synaptic clustering of receptors (Craig and Lichtman, 2001). The glycine receptor may also act in concert with an NMDAR subunit to form an excitatory receptor (Chatterton et al., 2002). Anti-Glycine Receptor Western blot of rat spinal cord showing specific immunolabeling of the ~48k (1- and (2-subunits of the glycine receptor. The labeling was absent from a rat hippocampal (hipp) lysate as the glycine receptor is not expressed in the hippocampus.

Poids moléculaire: '48 kDa

ID gène: 25674

UniProt: P07727

Pathways: Synaptic Membrane