SYN1 anticorps (pSer549)

N° du produit ABIN361492

Détail du produit anti-SYN1 anticorps

Antigène: SYN1 (Synapsin I (SYN1))

Épitope: pSer549

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SYN1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Specificité: Specific for ~78k synapsin I doublet phosphorylated at Ser549. Immunolabeling of the synapsin I band is blocked by (-phosphatase treatment.

Réactivité croisée: Rat (Rattus)

Homologie: bovine, canine, human, mouse, non-human primate

Purification: Antigen Affinity Purified from Pooled Serum

Immunogène: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser549 conjugated to KLH

Information d'application

Indications d'application: Recommended Dilution: WB: 1:1000 IHC: 1:500 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Stock: -20 °C

Références pour anticorps anti-SYN1 (ABIN361492)

Dagyte, Luiten, De Jager, Gabriel, Mocaër, Den Boer, Van der Zee: "Chronic stress and antidepressant agomelatine induce region-specific changes in synapsin I expression in the rat brain." dans: Journal of neuroscience research, Vol. 89, Issue 10, pp. 1646-57, (2011) (PubMed).

Détails sur SYN1

Antigène: SYN1 (Synapsin I (SYN1))

Autre désignation: SYN1 (SYN1 Produits)

Synonymes: anticorps SYN1a, anticorps SYN1b, anticorps SYNI, anticorps Syn-1, anticorps SYN I, anticorps si:dkey-90n12.3, anticorps synapsin I, anticorps synapsin I L homeolog, anticorps SYN1, anticorps Syn1, anticorps syn1.L, anticorps syn1

Sujet: Synapsin I plays a key role in synaptic plasticity in brain (Feng et al., 2002, Nayak et al., 1996). This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. The role of synapsin in synaptic plasticity and in synaptogensis is regulated by phosphorylation (Jovanovic et al., 2001, Kao et al., 2002). Ser 549 along with Ser 62 and Ser 67 are the sites of synapsin I that are phosphorylated by MAP kinase (Jovanovic et al., 1996). Phosphorylation and subsequent dephosphorylation of this site is thought to play a key role in synaptic vesicle trafficking. Anti-Phospho-Ser549 Synapsin Western blot of rat cortex lysate showing specific immunolabeling of the ~78k synapsin I phosphorylated at Ser549 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: (-Ptase). The blot is identical to the control except that it was incubated in (-Ptase (1200 units for 30 min) before being exposed to the phospho Ser549 synapsin I antibody. The immunolabeling is completely eliminated by treatment with (-Ptase.

Poids moléculaire: '78 kDa

ID gène: 281510

UniProt: P17599