Specific for the ~49k MEK5 protein phosphorylated at Ser311, Thr315. Immunolabeling of the MEK5 band is blocked by preadsorption with the phospho-peptide used as antigen but not be the corresponding dephospho-peptide.
Réactivité croisée
Rat (Rattus)
Homologie
bovine, canine, chicken, human, mouse, non-human primates, Xenopus, zebra fish
Purification
Antigen Affinity Purified from Pooled Serum
Immunogène
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser311/Thr315 conjugated to KLH
MEK5 (also known as MKK5) is a dual specificity serine/threonine protein kinase belonging to the MAP kinase kinase family. MEK5 has been shown to specifically activate ERK5 (Zhou et al., 1995) whereas MEK5 itself is regulated by MEKK3 (Chao et al., 1999). An important link between MEK5 and metastatic prostrate cancer has been demonstrated (Mehta et.al., 2003). Dual phosphorylation of Ser311 and Thr315 have been implicated in cell proliferation (Cameron et al., 2004). Anti-Phospho-Ser311, Thr315 MEK5 Western blot of rat testes lysate showing specific labeling of the ~49k MEK5 protein (Control). Immunolabeling is blocked by preadsorption with the phospho-peptide used as antigen (Peptide) but not by the corresponding dephospho-peptide (not shown).