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DNAJB1 anticorps

DNAJB1 Reactivité: Saccharomyces cerevisiae WB, ELISA, IP Hôte: Souris Monoclonal 2A7-H6 unconjugated
N° du produit ABIN361662
  • Antigène Voir toutes DNAJB1 Anticorps
    DNAJB1 (DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1))
    Reactivité
    • 77
    • 36
    • 31
    • 11
    • 8
    • 6
    • 6
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Saccharomyces cerevisiae
    Hôte
    • 57
    • 37
    Souris
    Clonalité
    • 57
    • 37
    Monoclonal
    Conjugué
    • 51
    • 7
    • 5
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp DNAJB1 est non-conjugé
    Application
    • 79
    • 41
    • 40
    • 39
    • 35
    • 22
    • 10
    • 6
    • 2
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunoprecipitation (IP)
    Specificité
    Detects ~40 kDa. Yeast specific. Does not cross react with Human, Mouse or Rat.
     Réactivité croisée
    Saccharomyces cerevisiae
    Purification
    Protein G Purified
    Immunogène
    Full length protein HSP40 (YDJ1)
    Clone
    2A7-H6
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product DNAJB1 Anticorps primaire
  • Indications d'application
    • WB (1:2000)
    • optimal dilutions for assays should be determined by the user.
    Commentaires

    0.5 μg/ml of ABIN361661 was sufficient for detection of 50 ng YDJ1 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    -20 °C
    Stockage commentaire
    -20°C
  • Knighton, Nitika, Waller, Strom, Wolfgeher, Reitzel, Truman: "Dynamic remodeling of the interactomes of Nematostella vectensis Hsp70 isoforms under heat shock." dans: Journal of proteomics, Vol. 206, pp. 103416, (2020) (PubMed).

    Sluder, Nitika, Knighton, Truman: "The Hsp70 co-chaperone Ydj1/HDJ2 regulates ribonucleotide reductase activity." dans: PLoS genetics, Vol. 14, Issue 11, pp. e1007462, (2019) (PubMed).

  • Antigène
    DNAJB1 (DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1))
    Autre désignation
    HSP40 (DNAJB1 Produits)
    Sujet
    Human HSP40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein) (1). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain (2). Members of the HSP40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. In particular, Hdj1, the first human HSP40/DnaJ protein identified, plays an important role in protein translation and folding, as well as in the regulation of HSP70 function (3). HSP40 stimulates the ATPase activity of HSP70 which in turn causes conformational changes of the unfolded proteins (4, 5). The HSP40-HSP70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1 (6). Some studies have shown that the difference between HDJ1 and type 1 DNAJ proteins including HDJ2 and yeast YdjI is the result of the possession of a zinc finger domain by the latter, which helps in the function of protein folding. (7, 8).
    ID gène
    855661
    NCBI Accession
    NP_014335
    UniProt
    P25491
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