TLR4 anticorps (AA 420-435)

N° du produit ABIN361724

Détail du produit anti-TLR4 anticorps

Antigène: TLR4 (Toll-Like Receptor 4 (TLR4))

Épitope: AA 420-435

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TLR4 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificité: Detects ~75-80 kDa when tested against partial recombinant mouse TLR4 (extra-cellular portion plus His-tag).

Réactivité croisée: Humain, Souris

Purification: Protein A Purified

Immunogène: Developed against a synthetic peptide corresponding to amino acids 420-435 of human TLR4

Information d'application

Indications d'application:

• WB (1:500)
• IHC (1:50)
• optimal dilutions for assays should be determined by the user.

Commentaires:

2 μg/ml of ABIN361724 was sufficient for detection of TLR4 in 100 ng of partial recombinant mouse TLR4 protein by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: -20°C

Références pour anticorps anti-TLR4 (ABIN361724)

Vercellotti, Dalmasso, Schaid, Nguyen, Chen, Ericson, Abdulla, Killeen, Lindorfer, Taylor, Belcher: "Critical role of C5a in sickle cell disease." dans: American journal of hematology, Vol. 94, Issue 3, pp. 327-337, (2019) (PubMed).

Nakano, Furube, Morita, Wanaka, Nakashima, Miyata: "Astrocytic TLR4 expression and LPS-induced nuclear translocation of STAT3 in the sensory circumventricular organs of adult mouse brain." dans: Journal of neuroimmunology, Vol. 278, pp. 144-58, (2015) (PubMed).

Takano, Ohkusa, Otani, Min, Kadoyama, Minami, Sano, Matsuyama: "Lipid A-activated inducible nitric oxide synthase expression via nuclear factor-κB in mouse choroid plexus cells." dans: Immunology letters, Vol. 167, Issue 2, pp. 57-62, (2015) (PubMed).

Détails sur TLR4

Antigène: TLR4 (Toll-Like Receptor 4 (TLR4))

Autre désignation: TLR4 (TLR4 Produits)

Synonymes: anticorps ARMD10, anticorps CD284, anticorps TLR-4, anticorps TOLL, anticorps Lps, anticorps Ly87, anticorps Ran/M1, anticorps Rasl2-8, anticorps toll like receptor 4, anticorps toll-like receptor 4, anticorps TLR4, anticorps Tlr4

Sujet: The Toll-like receptor (TLR) family in mammal comprises a family of trans-membrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and 1L-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylo-genetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). Amount this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signaling (4,5). The mouse TLR4 cDNA codes for a protein consisting of 839 amino acids, with an approximate molecular weight of 90 kDa (6).

ID gène: 7099

NCBI Accession: NP_612564

UniProt: O00206

Pathways: Signalisation TLR, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome, S100 Proteins