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CD74 anticorps

CD74 Reactivité: Humain FACS, WB, IF, IHC, IP, ICC Hôte: Souris Monoclonal PIN-1 unconjugated
N° du produit ABIN361789
  • Antigène Voir toutes CD74 Anticorps
    CD74 (HLA-DR-gamma (CD74))
    Reactivité
    • 186
    • 67
    • 13
    • 11
    Humain
    Hôte
    • 119
    • 87
    • 4
    • 2
    Souris
    Clonalité
    • 134
    • 76
    • 2
    Monoclonal
    Conjugué
    • 68
    • 16
    • 15
    • 10
    • 9
    • 7
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Cet anticorp CD74 est non-conjugé
    Application
    • 121
    • 109
    • 75
    • 39
    • 36
    • 34
    • 28
    • 27
    • 17
    • 14
    • 13
    • 11
    • 9
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
    Specificité
    Detects ~33-35 kDa protein doublet corresponding to the molecular mass of the p33 and p35 forms of human CD74.
     Réactivité croisée
    Humain, Souris
    Purification
    Protein G Purified
    Immunogène
    Human CD74 invariant chain synthetic peptide
    Clone
    PIN-1
    Isotype
    IgG1
    Top Product
    Discover our top product CD74 Anticorps primaire
  • Indications d'application
    • WB (1:1000)
    • IHC (1:100)
    • ICC/IF (1:50)
    • optimal dilutions for assays should be determined by the user.
    Commentaires

    1 μg/ml of ABIN361788 was sufficient for detection of CD74 in 20 μg of PALA cell lysates by colorimetric immunolot analysis using goat anti-mouse IgG: AP as the secondary antibody.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.2, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    -20 °C
    Stockage commentaire
    -20°C
  • Schneppenheim, Hüttl, Kruchen, Fluhrer, Müller, Saftig, Schneppenheim, Martin, Schröder: "Signal-peptide-peptidase-like 2a is required for CD74 intramembrane proteolysis in human B cells." dans: Biochemical and biophysical research communications, Vol. 451, Issue 1, pp. 48-53, (2014) (PubMed).

    Katsel, Tan, Haroutunian: "Gain in brain immunity in the oldest-old differentiates cognitively normal from demented individuals." dans: PLoS ONE, Vol. 4, Issue 10, pp. e7642, (2009) (PubMed).

  • Antigène
    CD74 (HLA-DR-gamma (CD74))
    Autre désignation
    CD74 (CD74 Produits)
    Synonymes
    anticorps DHLAG, anticorps HLADG, anticorps II, anticorps Ia-GAMMA, anticorps CLIP, anticorps Ii, anticorps INVG34, anticorps Iclp-1, anticorps iclp1, anticorps wu:fb11f09, anticorps wu:fb12a01, anticorps wu:fb13d06, anticorps wu:fk94e07, anticorps Iclp-2, anticorps cd74, anticorps iclp2, anticorps zgc:123161, anticorps CD74 molecule, anticorps CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated), anticorps CD74 molecule, major histocompatibility complex, class II invariant chain a, anticorps CD74 molecule, major histocompatibility complex, class II invariant chain b, anticorps CD74, anticorps Cd74, anticorps cd74a, anticorps cd74b
    Sujet
    CD74 is a non-polymorphic type II integral membrane protein. It has a short N-terminal cytoplasmic tail of 28 amino acids, followed by a single 24-aa transmembrane region and an approximately 150-aa lumenal domain (1). The CD74 chain is thought to function mainly as an MHC class II chaperone, which promotes ER exit of MHC class II molecules, directs them to endocytic compartments, prevents peptide binding in the ER, and contributes to peptide editing in the MHC class II compartment. Class II MHC and Ii expression was believed to be restricted to classical antigen-presenting cells (APC), however, during inflammation, other cell types, including mucosal epithelial cells, have also been reported to express class II MHC molecules (2). Experiments that investigate cell-surface CD74 are complicated by the fact that CD74 remains on the cell surface for a very short time. The surface half-life of CD74 was calculated to be fewer than 10 minutes (3). CD74 however has also recently been shown to have a role as an accessory-signaling molecule because of its high-affinity binding to the pro-inflammatory cytokine, macrophage migration-inhibitory factor (MIF) (3). The restricted expression of CD74 by normal tissues and its very rapid internalization make CD74 an attractive therapeutic target for both cancer and immunologic diseases (4).
    ID gène
    972
    NCBI Accession
    NP_001020329
    UniProt
    P04233
    Pathways
    Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Negative Regulation of intrinsic apoptotic Signaling, Cancer Immune Checkpoints
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