P4HB anticorps (AA 409-509)

N° du produit ABIN361829

Détail du produit anti-P4HB anticorps

Antigène: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Épitope: AA 409-509

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp P4HB est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificité: Detects ~58 kDa.

Réactivité croisée: Boeuf (Vache), Chien, Cobaye, Hamster, Humain, Mollusca, Souris, Porc, Rat, Mouton, Xenopus laevis

Purification: Peptide Affinity Purified

Immunogène: Rat PDI synthetic peptide (409-509 aa C-terminal) conjugated to KLH

Information d'application

Indications d'application:

• WB (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Commentaires:

A 1:1000 dilution of ABIN361828 was sufficient for detection of PDI in 20 μg of HeLa cell lysate by ECL immunoblot analysis.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: -20°C

Références pour anticorps anti-P4HB (ABIN361829)

Lauranzano, Pozzi, Pasetto, Stucchi, Massignan, Paolella, Mombrini, Nardo, Lunetta, Corbo, Mora, Bendotti, Bonetto: "Peptidylprolyl isomerase A governs TARDBP function and assembly in heterogeneous nuclear ribonucleoprotein complexes." dans: Brain : a journal of neurology, Vol. 138, Issue Pt 4, pp. 974-91, (2015) (PubMed).

Leung, Wang, Mak, Ng, Leung: "Differential proteomic responses in hepatopancreas and adductor muscles of the green-lipped mussel Perna viridis to stresses induced by cadmium and hydrogen peroxide." dans: Aquatic toxicology (Amsterdam, Netherlands), Vol. 105, Issue 1-2, pp. 49-61, (2011) (PubMed).

Détails sur P4HB

Antigène: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Autre désignation: PDI (P4HB Produits)

Sujet: The three dimensional structure of many extracellular proteins is stabilized by the formation of disulphide bonds. Studies suggest that a microsomal enzyme known as Protein Disulphide Isomerase (PDI) is involved in disulphide-bond formation via its oxidase activity and isomerization via its isomerase activity, as well as the reduction of disulphide bonds in proteins (1). Studies suggest BiP and PDI work together sequentially to increase oxidation of these proteins (2, 3). PDI has also been found to function as a chaperone to prevent the aggregation of unfolded substrates, and serves as a subunit of prolyl 4-hydroxylase and microsomal triglyceride transferase (4, 5). PDI is an abundant 55 kDa protein located primarily in the ER, however studies have also proved its presence in the cytosol (1). PDI has the ability to reside in the ER permanently due to the highly conserved KDEL sequence at its carboxy-terminus (6). It uses carboxy-terminal KDEL as a retention signal, and this appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor (7).

ID gène: 287164

NCBI Accession: NP_001099245

UniProt: P04785

Pathways: Maintenance of Protein Location, Cell RedoxHomeostasis, Lipid Metabolism