P4HB anticorps (AA 409-509)
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- Antigène Voir toutes P4HB Anticorps
- P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))
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Épitope
- AA 409-509
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Reactivité
- Rat
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp P4HB est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)
- Specificité
- Detects ~58 kDa.
- Réactivité croisée
- Boeuf (Vache), Chien, Cobaye, Hamster, Humain, Mollusca, Souris, Porc, Rat, Mouton, Xenopus laevis
- Purification
- Peptide Affinity Purified
- Immunogène
- Rat PDI synthetic peptide (409-509 aa C-terminal) conjugated to KLH
- Top Product
- Discover our top product P4HB Anticorps primaire
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- Indications d'application
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- WB (1:1000)
- ICC/IF (1:100)
- optimal dilutions for assays should be determined by the user.
- Commentaires
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A 1:1000 dilution of ABIN361828 was sufficient for detection of PDI in 20 μg of HeLa cell lysate by ECL immunoblot analysis.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- -20 °C
- Stockage commentaire
- -20°C
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Peptidylprolyl isomerase A governs TARDBP function and assembly in heterogeneous nuclear ribonucleoprotein complexes." dans: Brain : a journal of neurology, Vol. 138, Issue Pt 4, pp. 974-91, (2015) (PubMed).
: "Differential proteomic responses in hepatopancreas and adductor muscles of the green-lipped mussel Perna viridis to stresses induced by cadmium and hydrogen peroxide." dans: Aquatic toxicology (Amsterdam, Netherlands), Vol. 105, Issue 1-2, pp. 49-61, (2011) (PubMed).
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Peptidylprolyl isomerase A governs TARDBP function and assembly in heterogeneous nuclear ribonucleoprotein complexes." dans: Brain : a journal of neurology, Vol. 138, Issue Pt 4, pp. 974-91, (2015) (PubMed).
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- Antigène
- P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))
- Autre désignation
- PDI (P4HB Produits)
- Sujet
- The three dimensional structure of many extracellular proteins is stabilized by the formation of disulphide bonds. Studies suggest that a microsomal enzyme known as Protein Disulphide Isomerase (PDI) is involved in disulphide-bond formation via its oxidase activity and isomerization via its isomerase activity, as well as the reduction of disulphide bonds in proteins (1). Studies suggest BiP and PDI work together sequentially to increase oxidation of these proteins (2, 3). PDI has also been found to function as a chaperone to prevent the aggregation of unfolded substrates, and serves as a subunit of prolyl 4-hydroxylase and microsomal triglyceride transferase (4, 5). PDI is an abundant 55 kDa protein located primarily in the ER, however studies have also proved its presence in the cytosol (1). PDI has the ability to reside in the ER permanently due to the highly conserved KDEL sequence at its carboxy-terminus (6). It uses carboxy-terminal KDEL as a retention signal, and this appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor (7).
- ID gène
- 287164
- NCBI Accession
- NP_001099245
- UniProt
- P04785
- Pathways
- Maintenance of Protein Location, Cell RedoxHomeostasis, Lipid Metabolism
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