Calreticulin anticorps

N° du produit ABIN361835

Détail du produit anti-Calreticulin anticorps

Antigène: Calreticulin (CALR)

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Calreticulin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificité: Detects ~63 kDa.

Réactivité croisée: Poulet, Boeuf (Vache), Chien, Cobaye, Hamster, Humain, Singe, Souris, Porc, Lapin, Rat, Mouton

Purification: Peptide Affinity Purified

Immunogène: Human calreticulin synthetic peptide with a cysteine residue added and the peptide conjugated to KLH

Information d'application

Indications d'application:

• WB (1:1000)
• IHC (1:100)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Commentaires:

A 1:1000 dilution of ABIN361834 was sufficient for detection of Calreticulin in 20 μg of HeLa cell lysate by ECL immunoblot analysis.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: -20°C

Références pour anticorps anti-Calreticulin (ABIN361835)

Schultheis, Smith, Kiosses, Kraynyak, Wong, Oh, Broderick: "Delineating the Cellular Mechanisms Associated with Skin Electroporation." dans: Human gene therapy methods, Vol. 29, Issue 4, pp. 177-188, (2019) (PubMed).

Koide, Kondo, Odkhuu, Ulziisaikhan, Ukaji, Yokochi, Umezawa: "Inhibition of receptor activator of nuclear factor-κB ligand- or lipopolysaccharide-induced osteoclast formation by conophylline through downregulation of CREB." dans: Immunology letters, Vol. 161, Issue 1, pp. 31-7, (2014) (PubMed).

Kobayashi, Hoshinaga, Miura, Tokuda, Shigeoka, Murai, Horio: "Ascorbic acid deficiency decreases hepatic cytochrome P-450, especially CYP2B1/2B2, and simultaneously induces heme oxygenase-1 gene expression in scurvy-prone ODS rats." dans: Bioscience, biotechnology, and biochemistry, Vol. 78, Issue 6, pp. 1060-6, (2014) (PubMed).

Frenz, Graalmann, Detje, Döring, Grabski, Scheu, Kalinke et al.: "Independent of plasmacytoid dendritic cell (pDC) infection, pDC triggered by virus-infected cells mount enhanced type I IFN responses of different composition as opposed to pDC stimulated with free ..." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 193, Issue 5, pp. 2496-503, (2014) (PubMed).

Kluge, Mack, Iyer, Pujol, Heigele, Learn, Usmani, Sauter, Joas, Hotter, Bibollet-Ruche, Plenderleith, Peeters, Geyer, Sharp, Fackler, Hahn, Kirchhoff: "Nef proteins of epidemic HIV-1 group O strains antagonize human tetherin." dans: Cell host & microbe, Vol. 16, Issue 5, pp. 639-50, (2014) (PubMed).

Tongu, Harashima, Yamada, Harada, Harada: "Immunogenic chemotherapy with cyclophosphamide and doxorubicin against established murine carcinoma." dans: Cancer immunology, immunotherapy : CII, Vol. 59, Issue 5, pp. 769-77, (2010) (PubMed).

Détails sur Calreticulin

Antigène: Calreticulin (CALR)

Autre désignation: Calreticulin (CALR Produits)

Synonymes: anticorps CRT, anticorps RO, anticorps SSA, anticorps cC1qR, anticorps crc, anticorps crt, anticorps Calregulin, anticorps CG9429, anticorps Clr, anticorps Dmel\\CG9429, anticorps anon-EST:Liang-2.11, anticorps anon-EST:Liang-2.17, anticorps anon-EST:fe3D7, anticorps clone 2.11, anticorps clone 2.17, anticorps eth, anticorps potp, anticorps cc1qr, anticorps ssa, anticorps NV19007, anticorps CRP55, anticorps ERp60, anticorps HACBP, anticorps CHOT1, anticorps CHT1, anticorps CT1, anticorps AA589632, anticorps CRTR, anticorps Creat, anticorps CCDS1, anticorps calrl2, anticorps fc66d10, anticorps wu:fc66d10, anticorps wu:fj50c03, anticorps zgc:66153, anticorps calreticulin, anticorps calreticulin L homeolog, anticorps Calreticulin, anticorps putative calreticulin, anticorps solute carrier family 6 member 8, anticorps solute carrier family 6 (neurotransmitter transporter, creatine), member 8, anticorps CALR, anticorps calr.L, anticorps Crt, anticorps crt, anticorps CRT, anticorps Calr, anticorps calr, anticorps LMJF_31_2600, anticorps Crc, anticorps Slc6a8, anticorps SLC6A8, anticorps LOC8269812

Sujet: Calreticulin is a multifunctional, highly conserved Ca2+ -binding protein that is localized to the endoplasmic reticulum (ER), but has also been detected in the nucleus and nuclear envelop. Like many other ER proteins, it has the conserved ER retention KDEL (Lys-Asp-Glu-Leu) sequence at its C-terminus (1-3). CRT's three domains include a 180 residue N-terminal domain, a proline-rich P-domain (residues 189-288) that binds Ca2+ with high affinity and shares homology with calnexin (CNX) and calmegin, and a 110 residue C-terminal domain that binds Ca2+ with low affinity but high capacity (1,3). Recent studies suggest that this soluble ER protein has a multifunctional role. It appears to be involved in calcium storage and regulation as well as having a molecular chaperone activity. It has been shown to interact with the cytoskeleton and to be involved in the regulation of gene expression. Calreticulin may also play a role in cellular proliferation including its apparent activity in the proliferation of certain viruses within mammalian host cells (4, 5), and it has also been shown to be induced in response to various types of cell stress including amino acid deprivation (6). Close interconnections among protein synthesis, gene expression and calcium signaling have been observed by many researchers in recent years. Calreticulin might be centrally located and therefore it crucially participates in the coordination of many functions by the cell (4, 5). Studies also suggest its involvement in a few diseases such as systemic lupus erythematosus, rheumatoid arthritis, celiac disease, complete congenital heart block, and halothane hepatitis (1).

ID gène: 811

NCBI Accession: NP_004334

UniProt: P27797

Pathways: Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, ER-Nucleus Signaling, Unfolded Protein Response