The antibody detects endogenous level of STAT3 only when phosphorylated at tyrosine 705.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Immunogène
Peptide sequence around phosphorylation site of pTyr705 (A-P-Y (p) -L-K) derived from Human STAT3. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Western blotting: 1:500-1:1000 Immunohistochemistry: 1:50-1:100
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C/-20 °C
Stockage commentaire
Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Ma, Li, Wang, Gong, Zhang, Li, Ji, Liu, Chen, Xiang: "Atrial natriuretic peptide suppresses Th17 development through regulation of cGMP-dependent protein kinase and PI3K-Akt signaling pathways." dans: Regulatory peptides, Vol. 181, pp. 9-16, (2013) (PubMed).
Wang, Peng-Jiao, Yang, Chuang-Yuan, Zhao, Liu, Fei, Zhang: "PF-04691502 triggers cell cycle arrest, apoptosis and inhibits the angiogenesis in hepatocellular carcinoma cells." dans: Toxicology letters, Vol. 220, Issue 2, pp. 150-6, (2013) (PubMed).
Zhang, Zhao, Xin: "Significance and relationship between Cripto-1 and p-STAT3 expression in gastric cancer and precancerous lesions." dans: World journal of gastroenterology : WJG, Vol. 16, Issue 5, pp. 571-7, (2010) (PubMed).
Takeichi, Sugiura, Muro, Matsumoto, Ogawa, Futamura, Kaminuma, Hashimoto, Shimoyama, Saito, Tomita: "Overexpression of LEDGF/DFS70 induces IL-6 via p38 activation in HaCaT cells, similar to that seen in the psoriatic condition." dans: The Journal of investigative dermatology, Vol. 130, Issue 12, pp. 2760-7, (2010) (PubMed).
García-Prieto, González-López, Cabrera, Astudillo, Gutiérrez-Fernández, Fanjul-Fernandez, Batalla-Solís, Puente, Fueyo, López-Otín, Albaiceta: "Resistance to bleomycin-induced lung fibrosis in MMP-8 deficient mice is mediated by interleukin-10." dans: PLoS ONE, Vol. 5, Issue 10, pp. e13242, (2010) (PubMed).
Ren, Li, Xiao, Yan, Yang, Wang, Guo, Wang: "Endocrine glands-derived vascular endothelial growth factor protects pancreatic cancer cells from apoptosis via upregulation of the myeloid cell leukemia-1 protein." dans: Biochemical and biophysical research communications, Vol. 386, Issue 1, pp. 35-9, (2009) (PubMed).
Yuan, Qian, Shi, Lu, Li, Hu, Yu, Shen, Guo: "HER2-dependent MMP-7 expression is mediated by activated STAT3." dans: Cellular signalling, Vol. 20, Issue 7, pp. 1284-91, (2008) (PubMed).
Yamaguchi, Zhu, Yu, Sasaki, Umetsu, Kidachi, Ryoyama et al.: "Serum-free mouse embryo cells generate a self-sustaining feedback loop for an astrocyte marker protein and respond to cytokines and bisphenol A in accordance with the subtle difference in their ..." dans: Cell biology international, Vol. 31, Issue 6, pp. 638-44, (2007) (PubMed).
Yamaguchi, Zhu, Yu, Sasaki, Umetsu, Kidachi, Ryoyama: "Low-level bisphenol A increases production of glial fibrillary acidic protein in differentiating astrocyte progenitor cells through excessive STAT3 and Smad1 activation." dans: Toxicology, Vol. 226, Issue 2-3, pp. 131-42, (2006) (PubMed).
Antigène
STAT3
(Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3))