The antibody detects endogenous level of Caveolin-1 only when phosphorylated at tyrosine 14.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Immunogène
Peptide sequence around phosphorylation site of pTyr14 (H-L-Y (p) -T-V) derived from Human CAVEOLIN-1. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
CAV1
Reactivité: Humain, Rat, Souris
WB, ELISA, IHC, IF, ICC
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
Western blotting: 1:500-1:1000 Immunofluorescence: 1:100-1:200
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C/-20 °C
Stockage commentaire
Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Bento-Abreu, Velasco, Polo-Hernández, Lillo, Kozyraki, Tabernero, Medina: "Albumin endocytosis via megalin in astrocytes is caveola- and Dab-1 dependent and is required for the synthesis of the neurotrophic factor oleic acid." dans: Journal of neurochemistry, Vol. 111, Issue 1, pp. 49-60, (2009) (PubMed).
The scaffolding protein encoded by Caveolin-1 is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 MAP kinase cascade. CAV1 and CAV2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. By using alternative initiation codons in the same reading frame, two isoforms (a and beta) are encoded by a single transcript from this gene.