Cet anticorp Phospholipase C gamma 1 est non-conjugé
Application
Western Blotting (WB)
Specificité
The antibody detects endogenous level of PLCg1 only when phosphorylated at tyrosine 783.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Immunogène
Peptide sequence around phosphorylation site of pTyr783 (G-F-Y (p) -V-E) derived from Human PLCG1. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
PLCG1
Reactivité: Humain
WB, ELISA, IHC
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
Western blotting: 1:500-1:1000
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C/-20 °C
Stockage commentaire
Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Pan, Dong, Wang, Yang, Zhang, Li, Zhang, Yang, Li, Tan, Wang: "Signal regulatory protein ? negatively regulates mast-cell activation following Fc?RI aggregation." dans: European journal of immunology, Vol. 43, Issue 6, pp. 1598-607, (2013) (PubMed).
Hsieh, Liu, Hsu, Jayakumar, Chou, Wang, Hsiao, Sheu et al.: "Inhibition of vascular smooth muscle cell proliferation by the vitamin E derivative pentamethylhydroxychromane in an in vitro and in vivo study: pivotal role of hydroxyl radical-mediated PLCgamma1 ..." dans: Free radical biology & medicine, Vol. 49, Issue 5, pp. 881-93, (2010) (PubMed).
Antigène
Phospholipase C gamma 1 (PLCG1)
(phospholipase C, gamma 1 (PLCG1))