The antibody detects endogenous level of ATM only when phosphorylated at serine 1981.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Immunogène
Peptide sequence around phosphorylation site of pSer1981 (E-G-S (p) -Q-S) derived from Human ATM. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
ATM
Reactivité: Humain
IF, DB
Hôte: Lapin
Polyclonal
RB8121
unconjugated
Indications d'application
Western blotting: 1:500-1:1000 Immunohistochemistry: 1:50-1:100
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C/-20 °C
Stockage commentaire
Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Inaba, Kuboniwa, Sugita, Lamont, Amano: "Identification of signaling pathways mediating cell cycle arrest and apoptosis induced by Porphyromonas gingivalis in human trophoblasts." dans: Infection and immunity, Vol. 80, Issue 8, pp. 2847-57, (2012) (PubMed).
Mahalingam, Tay, Tan, Chai, Wang: "Mutant telomerase RNAs induce DNA damage and apoptosis via the TRF2-ATM pathway in telomerase-overexpressing primary fibroblasts." dans: The FEBS journal, Vol. 278, Issue 19, pp. 3724-38, (2011) (PubMed).
Leemput, Masson, Bigot, Errachid, Dansault, Provost, Gadin, Aoufouchi, Menasche, Abitbol: "ATM localization and gene expression in the adult mouse eye." dans: Molecular vision, Vol. 15, pp. 393-416, (2009) (PubMed).
Kang, Guo, Tan, Zhao, Tang, Lu: "Expression status of ataxia-telangiectasia-mutated gene correlated with prognosis in advanced gastric cancer." dans: Mutation research, Vol. 638, Issue 1-2, pp. 17-25, (2008) (PubMed).
ATM encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates, thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. Two transcript variants encoding different isoforms have been found for this gene.