The antibody detects endogenous level of HDAC5 only when phosphorylated at serine498.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Immunogène
Peptide sequence around phosphorylation site of pSer498 (T-Q-S (p) -S-P) derived from Human HDAC5, 7. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
HDAC5
Reactivité: Humain
IHC, ELISA, IF
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
Western blotting: 1:500-1:1000 Immunohistochemistry: 1:50-1:100
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C/-20 °C
Stockage commentaire
Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Wang, Ha, Jhun, Wong, Jain, Jin: "Fluid shear stress stimulates phosphorylation-dependent nuclear export of HDAC5 and mediates expression of KLF2 and eNOS." dans: Blood, Vol. 115, Issue 14, pp. 2971-9, (2010) (PubMed).
Xia, Li, Johnson, Seidel, Wallace, Li: "Polycystin-dependent fluid flow sensing targets histone deacetylase 5 to prevent the development of renal cysts." dans: Development (Cambridge, England), Vol. 137, Issue 7, pp. 1075-84, (2010) (PubMed).
Borok, Tran, Ho, Drewell: "Dissecting the regulatory switches of development: lessons from enhancer evolution in Drosophila." dans: Development (Cambridge, England), Vol. 137, Issue 1, pp. 5-13, (2009) (PubMed).
Bossuyt, Helmstadter, Wu, Clements-Jewery, Haworth, Avkiran, Martin, Pogwizd, Bers: "Ca2+/calmodulin-dependent protein kinase IIdelta and protein kinase D overexpression reinforce the histone deacetylase 5 redistribution in heart failure." dans: Circulation research, Vol. 102, Issue 6, pp. 695-702, (2008) (PubMed).
Pang, Yan, Natarajan, Cavet, Massett, Yin, Berk: "GIT1 mediates HDAC5 activation by angiotensin II in vascular smooth muscle cells." dans: Arteriosclerosis, thrombosis, and vascular biology, Vol. 28, Issue 5, pp. 892-8, (2008) (PubMed).
Lim, Luo, Koh, Yang, bin Abdul Kadir, Tan, Ye, Wang, Melamed et al.: "Distinct mechanisms involving diverse histone deacetylases repress expression of the two gonadotropin beta-subunit genes in immature gonadotropes, and their actions are overcome by ..." dans: Molecular and cellular biology, Vol. 27, Issue 11, pp. 4105-20, (2007) (PubMed).
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by HDAC5 belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. This gene is thought to be associated with colon cancer. Two transcript variants encoding different isoforms have been found for this gene. Doppler H, et al. (2005) J Biol Chem. 280(15):15013-15019. McKinsey TA, et al. (2000) Nature. 408(6808): 106-111. Published Papers J Bossuyt, K Helmstadter, X Wu, et al. Ca2+/Calmodulin-Dependent Protein Kinase II {delta} and Protein Kinase D Overexpression Reinforce. Circulation Research, 102(6):695-702.(2008) Pang J, Yan C, Natarajan K, et al. GIT1 mediates HDAC5 activation by angiotensin II in vascular smooth muscle cells.Arteriosclerosis, Thrombosis, and Vascular Biology, 28(5):892-8. (2008) S Lim, M Luo, M Koh, et al. Distinct Mechanisms Involving Diverse Histone Deacetylases Repress Expression of the Two Gonadotropin beta-Subunit Genes in Immature Gonadotropes, and Their Actions Are Overcome by Gonadotropin-Releasing Hormone.