The antibody detects endogenous level of total Myc protein.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Immunogène
Peptide sequence around AA 356-360 (R-R-T-H-N) derived from Human Myc. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
MYC
Reactivité: Humain, Souris, Rat
WB, IHC, ELISA, IF, ICC
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
Western blotting: 1:500-1:1000 Immunohistochemistry: 1:50-1:100
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C/-20 °C
Stockage commentaire
Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Giugliano, Ruff, Braunwald, Murphy, Wiviott, Halperin, Waldo, Ezekowitz, Weitz, Špinar, Ruzyllo, Ruda, Koretsune, Betcher, Shi, Grip, Patel, Patel, Hanyok, Mercuri, Antman: "Edoxaban versus warfarin in patients with atrial fibrillation." dans: The New England journal of medicine, Vol. 369, Issue 22, pp. 2093-104, (2013) (PubMed).
Liu, Ma, Lee: "NMDA receptor signaling mediates the expression of protein inhibitor of activated STAT1 (PIAS1) in rat hippocampus." dans: Neuropharmacology, Vol. 65, pp. 101-13, (2012) (PubMed).
Myc proto-oncogene encodes nuclear DNA-binding phosphoproteins that are involved in the regulation of gene expression and DNA replication during cell growth and differentiation. Myc encodes a protein of 65 kDa which is expressed in almost all normal and transformed cells. The expression correlates with the proliferation state of the cells. Transcription is repressed in quiescent or terminally differentiated cells. Expression of Myc is generally induced after mitogenic stimulation of cells or serum induction. Myc therefore is an important positive regulator of cell growth and proliferation. Myc has been demonstrated also to be a potent inducer of apoptosis when expressed in the absence of serum or growth factors. Apoptosis may serve also as a protective mechanism to prevent tumorigenicity elicited by deregulated Myc expression. Sequences of the Myc oncogene have been highly conserved throughout evolution, from Drosophila to vertebrates