Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Specificité
The antibody detects endogenous level of total 4E-BP1 protein.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen.
Immunogène
Peptide sequence around AA 43-47 (S-T-T-P-G) derived from Human 4E-BP1. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
4E-BP1 encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.