The antibody detects endogenous level of total IRS-1 protein.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen.
Immunogène
Peptide sequence around AA 637-641 (P-K-S-V-S) derived from Human IRS-1. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
IRS1
Reactivité: Humain, Souris, Rat
WB, IHC, ELISA, IF
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
Western blotting: 1:500-1:1000 Immunofluorescence: 1:100-1:200
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C/-20 °C
Stockage commentaire
Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Ekuni, Tomofuji, Irie, Kasuyama, Umakoshi, Azuma, Tamaki, Sanbe, Endo, Yamamoto, Nishida, Morita: "Effects of periodontitis on aortic insulin resistance in an obese rat model." dans: Laboratory investigation; a journal of technical methods and pathology, Vol. 90, Issue 3, pp. 348-59, (2010) (PubMed).
May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit