The antibody detects endogenous level of total MARCKS protein.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen.
Immunogène
Peptide sequence around AA 156-160 (R-F-S-F-K) derived from Human MARCKS. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
MARCKS
Reactivité: Rat
ELISA, IF (cc), IF (p), IHC (p), IHC (fro)
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
Western blotting: 1:500-1:1000 Immunofluorescence: 1:100-1:200
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C/-20 °C
Stockage commentaire
Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Olianas, Dedoni, Onali: "Signaling pathways mediating phosphorylation and inactivation of glycogen synthase kinase-3? by the recombinant human ?-opioid receptor stably expressed in Chinese hamster ovary cells." dans: Neuropharmacology, Vol. 60, Issue 7-8, pp. 1326-36, (2011) (PubMed).
Antigène
MARCKS
(Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))
anticorps 80K-L, anticorps MACS, anticorps PKCSL, anticorps PRKCSL, anticorps KINC, anticorps Macs, anticorps marcks, anticorps MGC89127, anticorps MARCKS, anticorps macs, anticorps wu:fb55c06, anticorps wu:fb92d04, anticorps wu:fc19b04, anticorps wu:fc44a10, anticorps zgc:110235, anticorps fc34g03, anticorps fc38c06, anticorps wu:fc34g03, anticorps wu:fc38c06, anticorps zgc:109978, anticorps myristoylated alanine rich protein kinase C substrate, anticorps myristoylated alanine-rich protein kinase C substrate, anticorps methyl binding domain106, anticorps myristoylated alanine-rich protein kinase C substrate b, anticorps myristoylated alanine-rich protein kinase C substrate S homeolog, anticorps myristoylated alanine-rich protein kinase C substrate a, anticorps MARCKS, anticorps Marcks, anticorps marcks, anticorps mbd106, anticorps marcksb, anticorps marcks.S, anticorps marcksa
Sujet
MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.