HIF1A anticorps (AA 432-528)

N° du produit ABIN363203

Détail du produit anti-HIF1A anticorps

Antigène: HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))

Épitope: AA 432-528

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Porc, Singe, Mouton, Furet

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp HIF1A est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS), Chromatin Immunoprecipitation (ChIP), Gel Shift (GS)

Specificité: Reacts with human HIF-1 alpha

Réactivité croisée: Souris, Boeuf (Vache), Lapin, Mouton

Réactivité croisée (Details): Cross reacts with proteins from monkey, sheep, mouse, rat, rabbit, pig, bovine and ferret.

Purification: Purified (protein A)

Immunogène: Fusion protein containing amino acids 432-528 of human HIF-1alpha

Clone: H1alpha67

Isotype: IgG2b

Information d'application

Indications d'application:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only:
IHC1:100-1:300 WB1:500-1:1000 ICC/ IF1:100-1:500 IP1:10

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.5 mg/mL

Buffer: Tris-glycine, 150 mM NaCl pH 7.5, Sodium azide 0.05 %

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store at 4°C. Do not freeze.

Détails sur HIF1A

Antigène: HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))

Autre désignation: Hypoxia-Inducible Factor 1-alpha (HIF1A Produits)

Synonymes: anticorps hif1a, anticorps AA959795, anticorps HIF1alpha, anticorps MOP1, anticorps bHLHe78, anticorps HIF-1A, anticorps HIF-1alpha, anticorps HIF1, anticorps HIF1-ALPHA, anticorps PASD8, anticorps HIF1-alpha, anticorps hif-1a, anticorps hypoxia inducible factor 1 alpha, anticorps hypoxia inducible factor 1, alpha subunit, anticorps hypoxia inducible factor 1 alpha subunit, anticorps HIF (Hypoxia Inducible Factor) homologa, anticorps hif-1a, anticorps Hif1a, anticorps HIF1A, anticorps hif1a, anticorps hif-1

Sujet: HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as a posttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes.

ID gène: 3091

UniProt: Q16665

Pathways: Positive Regulation of Peptide Hormone Secretion, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Transition Metal Ion Homeostasis, Tube Formation, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals, L'effet Warburg