Adenosine A2a Receptor anticorps (AA 373-391)

N° du produit ABIN364458

Détail du produit anti-Adenosine A2a Receptor anticorps

Antigène: Adenosine A2a Receptor (ADORA2A)

Épitope: AA 373-391

Reactivité: Humain, Chien

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Adenosine A2a Receptor est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Marque: IHC-plus™

Specificité: Detects adenosine receptor A2a. This antibody does not detect other AR subtypes. Species cross-reactivity: human. Not yet tested in other species.

Homologie: Percent identity by BLAST analysis: Dog (100%) Gorilla, Marmoset, Panda (89%) Human, Horse (84%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide: ESHGDMGLPDVELLSHELK, corresponding to amino acids 373-391 of Dog Adenosine Receptor A2a. Percent identity by BLAST analysis: Dog (100%), Gorilla, Marmoset, Panda (89%), Human, Horse (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (1:50), IP, WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:50. Western Blot: 1:1000 detects an ~45kD protein from canine striatum representing A2aAR. Immunohistochemistry (paraffin-embedded sections): 1:100. Staining of A2aAR in human hippocampus yields a pattern consistent with plasma membrane staining.

Commentaires:

Target Species of Antibody: Dog

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 1 mg/mL BSA, 0.05 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: 4°C or -20°C, Avoid freeze-thaw cycles.

Détails sur Adenosine A2a Receptor

Antigène: Adenosine A2a Receptor (ADORA2A)

Autre désignation: ADORA2A/Adenosine A2A Receptor (ADORA2A Produits)

Synonymes: anticorps ADORA2A, anticorps adora2a.1, anticorps zgc:194773, anticorps A2aR, anticorps ADORA2, anticorps RDC8, anticorps A2AAR, anticorps AA2AR, anticorps A2ar, anticorps ADENO, anticorps Adora2l1, anticorps adora2a.2, anticorps zgc:193802, anticorps zgc:193822, anticorps adenosine A2a receptor, anticorps adenosine A2a receptor a, anticorps adenosine A2a receptor L homeolog, anticorps adenosine A2a receptor b, anticorps ADORA2A, anticorps Adora2a, anticorps adora2aa, anticorps adora2a.L, anticorps adora2a, anticorps adora2ab

Sujet: Name/Gene ID: ADORA2A
Subfamily: Adenosine
Family: GPCR

Synonyms: ADORA2A, Adenosine receptor A2a, ADORA2, A2a receptor, Adenosine A2a receptor, Adenosine A2 receptor, Adenosine receptor subtype A2a, HA2aR, RDC8

ID gène: 135

UniProt: P29274

Pathways: Neurotrophin Signaling Pathway, cAMP Metabolic Process, Synaptic Membrane, Feeding Behaviour, Cancer Immune Checkpoints