Flow Cytometry: < / = 1 μg/10^6 cells. Immunohistochemistry on Frozen Sections. (Ref.13)Immunoprecipitation. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added.
Stock
4 °C/-20 °C
Stockage commentaire
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
CD11b (Integrin alpha M subunit) combines with CD18 (Integrin beta2 subunit) to form the integrin Mac-1, also known as complement receptor 3 (CR3), which mediates adhesion to C3bi and ICAM-1 (CD54). (Ref.2-6) Mac-1 is expressed at varying levels on granulocytes, macrophages, dendritic cells, NK cells, and B-1 cells in the peritoneal and pleural cavities. (Ref.2,7-10) In addition to its role in binding C3bi on opsonized targets and mediation of the subsequent ingestion process. (Ref.4,11) Mac-1 is important as an adhesion molecule in the transendothelial migration of monocytes and neutrophils. (Ref.12)Synonyms: CD11 antigen-like family member B, CR-3 alpha chain, CR3A, Cell surface glycoprotein MAC-1 subunit alpha, ITGAM, Integrin alpha-M, Leukocyte adhesion receptor MO1, MAC1, Neutrophil adherence receptor