Cet anticorp Progesterone Receptor est non-conjugé
Application
Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))
Specificité
This antibody is specific for the ~90k PR-A isoform and the ~120k PR-B isoform phosphorylated at Ser294. Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.
Réactivité croisée (Details)
Species reactivity (expected):Mouse, Rat and non-Human Primates. Species reactivity (tested):Human.
Attributs du produit
Synonyms: PR, PGR, NR3C3, Nuclear Receptor Subfamily 3 Group C Member 3
Purification
Affinity Chromatography on Protein G.
Immunogène
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser294of Human progesterone receptor.
Western Blot: 1/1000. Immunohistochemitry (Frozen Sections, unpublished observations): 1/1000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Buffer
10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.
Stock
-20 °C
Stockage commentaire
Store the antibody undiluted (in aliquots) at -20 °C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
There is accumulating evidence to suggest that progesterone plays an essential role in the regulation of growth and differentiation of mammary glands and thus may play a key role in breast cancer (Edwards, 2005). The biological response to progesterone is mediated by two distinct forms of the human progesterone receptor (PR-A and PR-B forms). In most cell contexts, the B form functions as a transcriptional activator, whereas the A form functions as a transcriptional inhibitor of steroid hormones (Attia et al., 2000, Lin et al., 2003). Recently it has been demonstrated that there is differential hormone dependent regulation of the phosphorylation of the A and B forms of the receptor (Clemm et al., 2000) . Treatment of T47D breast cancer cells with progestin agonist increases the phosphorylation of Ser190 and Ser294 with different kinetics. These phosphorylation events may differentially affect the transcriptional activity of the receptor.Synonyms: NR3C3, Nuclear Receptor Subfamily 3 Group C Member 3, PGR, PR