Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Dot Blot (DB)
Specificité
This antibody recognizes the ~48k alpha-1 and alpha-2 Subunits of the Glycine Receptor in Western blots of rat spinal cord and brain stem and in cell extracts. Immunolabeling blocked by preadsorption of antibody with the peptide immunogen. Does not recognize other Glycine Receptor subunits.
Réactivité croisée (Details)
Species reactivity (tested):Human, Mouse and Rat.
Purification
Affinity Chromatography.
Immunogène
Peptide from the N-terminus region of the alpha-1 subunit of rat Glycine Receptor.
GLRA1
Reactivité: Humain
WB
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
Dot blot: 1/1000. Western blot: 1/1000. Immunohistochemistry: 1/1000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore in 100 μL PBS (137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use.
Buffer
5 mM Ammonium Bicarbonate.
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
-20 °C
Stockage commentaire
After reconstitution: Store the antibody undiluted (in aliquots) at-20 °C.
Glycine is an important inhibitory transmitter in the brainstem and spinal cord. Glycine receptors are members of the ligand-gated ion channel family (LGICs) that mediate rapid chemical neurotransmission (Schofield et al., 2003). The binding of glycine to its receptor produces a large increase in chloride conductance, which causes membrane hyperpolarization. Glycine receptors are anchored at inhibitory chemical synapses by a cytoplasmic protein, gephyrin (Fischer et al., 2000). The glycine receptor has been used to great advantage in the identification of the binding sites for alcohol on the LGIC family of proteins (Beckstead et al., 2001, Mihic et al., 1997). These receptors have also been extremely useful in studies of synaptic clustering of receptors (Craig and Lichtman, 2001). The glycine receptor may also act in concert with an NMDAR subunit to form an excitatory receptor (Chatterton et al., 2002).Synonyms: GLRA1, Glycine receptor 48 kDa subunit, Glycine receptor strychnine-binding subunit