Parkin anticorps (pSer378)

N° du produit ABIN372698

L’anticorps anti-Parkin Polyclonal Lapin est utilisé pour la détection de Parkin dans des échantillons de Humain. Il a été validé pour WB.

Aperçu rapide pour Parkin anticorps (pSer378) (ABIN372698)

Antigène: Parkin (PARK2) (Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Parkin est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-Parkin anticorps

Épitope: pSer378

Specificité: This antibody recognizes the ~52k Parkin protein phosphorylated at Ser378. Immunolabeling of the parkin band is absent in parkin S378 mutants.

Réactivité croisée (Details): Species reactivity (tested):Human.

Purification: Sequential Chromatography on phospho- and dephosphopeptide affinity columns.

Immunogène: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser378 of human Parkin.

Isotype: IgG

Information d'application

Indications d'application: Western blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody undiluted (in aliquots) at-20 °C.

Détails sur Parkin

Antigène: Parkin (PARK2) (Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2))

Autre désignation: PARK2 / Parkin

Sujet: Parkin is an E3 ligase in the ubiquitin-proteasome system. Hereditary Parkinson's disease is most commonly caused by mutations in the parkin gene and is characterized by the progressive loss of dopaminergic neurons and the presence of Lewy bodies in the substania nigra (Jenner et al.,1992). Recent evidence suggests that phosphorylation of parkin at Ser378 may have an important regulatory role on its E3 ubiquitin ligase activity (Yamamoto et al., 2005).Synonyms: AR-JP, E3 ubiquitin-protein ligase parkin, LPRS2, PDJ, PRKN, Parkinson disease 2, Parkinson juvenile disease protein 2

ID gène: 5071

NCBI Accession: NP_004553

UniProt: O60260

Pathways: Autophagy, Ubiquitin Proteasome Pathway