RPH3A anticorps (pSer234)
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- Antigène Voir toutes RPH3A Anticorps
- RPH3A (Rabphilin 3A (RPH3A))
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Épitope
- pSer234
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Reactivité
- Souris, Rat
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp RPH3A est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Specificité
- This antibody recognizes the ~82k Rabphilin protein phosphorylated at Ser234. The immunolabeling of the Rabphilin band was completely blocked by the phosphopeptide used as antigen while the dephosphopeptide had no effect on the immunolabeling.
- Réactivité croisée (Details)
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Species reactivity (expected):Mouse.
Species reactivity (tested):Rat. - Purification
- Sequential Chromatography on phospho- and dephosphopeptide affinity columns.
- Immunogène
- Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser234 of Rabphilin 3A.
- Isotype
- IgG
- Top Product
- Discover our top product RPH3A Anticorps primaire
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- Indications d'application
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Western blot: 1/1,000. Immunohistochemistry: 1/400.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.
- Conseil sur la manipulation
- Avoid repeated freezing and thawing.
- Stock
- -20 °C
- Stockage commentaire
- Store the antibody undiluted (in aliquots) at-20 °C.
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- Antigène
- RPH3A (Rabphilin 3A (RPH3A))
- Autre désignation
- Rabphilin-3A / RPH3A (RPH3A Produits)
- Synonymes
- anticorps 2900002P20Rik, anticorps AU022689, anticorps AW108370, anticorps CG11556, anticorps Dmel\\CG11556, anticorps exophilin-1, anticorps fc61h10, anticorps rph3a, anticorps wu:fc61h10, anticorps rabphilin 3A, anticorps Rabphilin, anticorps rabphilin-3a, putative, anticorps rabphilin-3A, anticorps rabphilin 3A S homeolog, anticorps rabphilin 3A homolog (mouse), a, anticorps RPH3A, anticorps Rph3a, anticorps Rph, anticorps Smp_145120, anticorps Tsp_00335, anticorps rph3a.S, anticorps rph3aa
- Sujet
- Rabphilin-3A is a peripheral membrane protein that binds Ca2+ and phospholipids and is attached to synaptic vesicle membranes (Geppert et al., 1994, Li et al., 1994). The expression of rabphilin 3A is reduced in an animal model of Huntington's disease(Smith et al., 2005). Rabphilin can be phosphorylated at Ser234 by both CAM kinase II and PKA and this phosphorylation has been suggested to regulate neuronal activity during development in a synapse-specific manner (Fykse et al., 1995,Foletti and Scheller, 2001).Synonyms: Exophilin 1, Exophilin-1, KIAA0985, Rabphilin 3A
- ID gène
- 171039
- NCBI Accession
- NP_598202
- UniProt
- P47709
- Pathways
- Synaptic Vesicle Exocytosis
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