RAF1 anticorps (pSer301)
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- Antigène Voir toutes RAF1 Anticorps
- RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))
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Épitope
- pSer301
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Reactivité
- Humain, Souris, Rat, Boeuf (Vache), Poulet, Chien, Xenopus laevis, Non-Human Primate
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp RAF1 est non-conjugé
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Application
- Western Blotting (WB)
- Specificité
- This antibody recognizes the ~74k Raf-1 protein phosphorylated at Ser301. Immunolabeling of the Raf-1 protein band is blocked by l-phosphatase treatment.
- Réactivité croisée (Details)
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Species reactivity (expected):Human, Bovine, Canine, Mouse, Xenopus and non-Human Primates.
Species reactivity (tested):Rat. - Purification
- Sequential Chromatography on phospho- and dephosphopeptide affinity columns.
- Immunogène
- Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser301 of rat Raf1.
- Isotype
- IgG
- Top Product
- Discover our top product RAF1 Anticorps primaire
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- Indications d'application
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Western blot: 1/1,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.
- Conseil sur la manipulation
- Avoid repeated freezing and thawing.
- Stock
- -20 °C
- Stockage commentaire
- Store the antibody undiluted (in aliquots) at-20 °C.
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- Antigène
- RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))
- Autre désignation
- RAF1 (RAF1 Produits)
- Synonymes
- anticorps CRAF, anticorps NS5, anticorps Raf-1, anticorps c-Raf, anticorps c-raf, anticorps craf, anticorps raf1, anticorps RAF1, anticorps 6430402F14Rik, anticorps AA990557, anticorps BB129353, anticorps Craf1, anticorps D830050J10Rik, anticorps v-Raf, anticorps raf, anticorps cRaf, anticorps Raf-1 proto-oncogene, serine/threonine kinase, anticorps Raf-1 proto-oncogene, serine/threonine kinase a, anticorps v-raf-leukemia viral oncogene 1, anticorps Raf-1 proto-oncogene, serine/threonine kinase S homeolog, anticorps RAF1, anticorps Raf1, anticorps raf1a, anticorps raf1, anticorps raf1.S
- Sujet
- The Ras pathway is a critical signal transduction cascade involved in regulating cellular proliferation, differentiation, survival, and oncogenic transformation. Members of the Raf serine/threonine kinase family are key intermediates in this cascade, functioning to relay signals from activated Ras to the downstream protein kinases MEK and ERK (Marshall, 1996). Previous studies have shown that phosphorylation is required for Raf-1 activation (Dhillon and Kolch, 2002, Chong et al., 2003). Recent work has demonstrated that phosphorylation also regulates the downregulation of Raf (Dougherty et al., 2005) with two sites participating: Ser301 and Ser642.Synonyms: C-RAF, RAF proto-oncogene serine/threonine-protein kinase, Raf-1, cRaf
- ID gène
- 24703
- NCBI Accession
- NP_036771
- UniProt
- P11345
- Pathways
- Signalisation MAPK, Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling, BCR Signaling
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