DRAM anticorps (C-Term)

N° du produit ABIN372924

Détail du produit anti-DRAM anticorps

Antigène: DRAM (DRAM1) (DNA-Damage Regulated Autophagy Modulator 1 (DRAM1))

Épitope: C-Term

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DRAM est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: This antibody recognizes Damage-regulated Autophagy Modulator (DRAM).

Réactivité croisée (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography.

Immunogène: Synthetic peptide - KLH conjugated corresponding to a 16 amino acids peptide from near the carboxy terminus of Human DRAM.

Isotype: IgG

Information d'application

Indications d'application: ELISA. Western blot: 0.5-1.0 μg/mL. Immunohistochemistry on Frozen Sections: 2.5 μg/mL. Immunohistochemistry on Paraffin Sections: 3 μg/mL. This antibody was validated for use in immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody LS-D1, followed by alkalinephosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.02 % Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Détails sur DRAM

Antigène: DRAM (DRAM1) (DNA-Damage Regulated Autophagy Modulator 1 (DRAM1))

Autre désignation: DRAM1 (DRAM1 Produits)

Synonymes: anticorps DRAM1, anticorps DRAM, anticorps 1200002N14Rik, anticorps Dram, anticorps dram, anticorps DNA damage regulated autophagy modulator 1, anticorps DNA-damage regulated autophagy modulator 1, anticorps DNA damage regulated autophagy modulator 1 L homeolog, anticorps DRAM1, anticorps Dram1, anticorps dram1.L

Sujet: Damage-regulated autophagy modulator (DRAM) is a p53 target gene encoding a lysosomal protein that induces autophagy, a process that degrades cytosolic proteins and organelles. It has been suggested that activation of DRAM by p53 is simultaneous to the activation by p53 of one or more proapoptotic genes such as PUMA, Bax, etc., and that the signaling pathways regulated by these genes promote a full cell death response. By itself, DRAM cannot induce apoptosis, but the fact that it is inactivated in certain cancers highlights the importance of DRAM and suggests that autophagy may play a more important role in cancer than initially suspected. At least two different isoforms of DRAM are known to exist.Synonyms: DNA damage regulated autophagy modulator protein 1, DRAM, DRAM-1, FLJ11259

ID gène: 55332

NCBI Accession: NP_060840

UniProt: Q8N682