Cortactin anticorps (N-Term)

N° du produit ABIN374140

Cet anticorps Chèvre Polyclonal détecte spécifiquement Cortactin dans WB. Il présente une réactivité avec des échantillons de Humain, Souris, Rat, Chien et Boeuf (Vache).

Aperçu rapide pour Cortactin anticorps (N-Term) (ABIN374140)

Antigène: Cortactin (CTTN)

Reactivité: Humain, Souris, Rat, Chien, Boeuf (Vache)

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Cortactin est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-Cortactin anticorps

Épitope: N-Term

Séquence: WKASAGHAVS IA-C

Specificité: This antibody detects Cortactin at N-term.

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogène: Peptide from N Terminus of the protein sequence according to NP_005222.2, NP_612632.1 Genename: CTTN

Information d'application

Indications d'application: Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.01-0.03 μg/mL. Approx.80 kDa band observed in Mouse and Rat Brainlysates (calculated MW of 61.0 kDa according to Human NP_005222.2 and of 61.0 kDaaccording to Mouse NP_031829.2). The observed molecular weight corresponds to earlierfindings in literature with different antibodies (Paradis et al, J Cell Sci.2008 Jun 15,121(Pt12): 1965-72, PMID 18495841).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Détails sur Cortactin

Antigène: Cortactin (CTTN)

Autre désignation: Cortactin

Sujet: Cortactin is an 80/85 kDa cytoskeleton protein that facilitates assembly of cortical actin. Cortactin is widely expressed in most adherent cells and is a prominent substrate of protein tyrosine kinase Src in vivo and in vitro. The protein sequence of cortactin contains multiple amino terminal tandem repeats of a unique 37 amino acid sequence, which associates with actin, and a Src homology 3 (SH3) domain at the carboxyl terminus. Between the repeat and the SH3 domain, there is an alpha helical structure followed by a proline rich region. Between the proline rich region and the SH3 domain, there are a series of Src phosphorylation sites (Tyr421, Tyr470, and Tyr486). In vitro, cortactin binds to and cross links F actin into meshworks.Synonyms: Amplaxin, CTTN, EMS1, Oncogene EMS1

ID gène: 2017

NCBI Accession: NP_005222

UniProt: Q14247

Pathways: Signalisation MAPK