DKK4 anticorps (C-Term)
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- Antigène Voir toutes DKK4 Anticorps
- DKK4 (Dickkopf Homolog 4 (Xenopus Laevis) (DKK4))
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Épitope
- C-Term
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Reactivité
- Humain
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Hôte
- Chèvre
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp DKK4 est non-conjugé
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Application
- Western Blotting (WB)
- Séquence
- RQHARLRVCQ KIEKL
- Specificité
- This antibody detects Dickkopf-4 at C-term.
- Purification
- Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
- Immunogène
- Peptide from C Terminus of the protein sequence according to NP_055235.1 Genename: DKK4
- Top Product
- Discover our top product DKK4 Anticorps primaire
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- Indications d'application
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Peptide ELISA: Limit dilution 1: 32000. Western blot: 1-3 μg/mL. Approx 30-35 kDa band seen in Jurkat lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid repeated freezing and thawing.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
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- Antigène
- DKK4 (Dickkopf Homolog 4 (Xenopus Laevis) (DKK4))
- Autre désignation
- Dickkopf-4 (DKK4) (DKK4 Produits)
- Synonymes
- anticorps DKK-4, anticorps Dkk-4, anticorps RGD1563172, anticorps dickkopf WNT signaling pathway inhibitor 4, anticorps DKK4, anticorps Dkk4
- Sujet
- DKK4, like DKK1, DKK2, and DKK3, possesses an N terminal signal peptide and 2 conserved cysteine-rich domains, which are separated by a linker region and contain 10 cysteine residues each. The second cysteine region has a putative lipid-binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin-type proteases. Northern blot analysis detected no expression of DKK4, but RT-PCR analysis detected DKK4 expression in cerebellum, T cell, esophagus, and lung cDNA libraries. DKK4 blocks Xenopus Wnt8, Wnt3a, and Wnt2b, but not Dsh or Fz8, induction of a secondary axis in frog embryos, indicating that DKKs antagonize WNT function upstream of WNT receptors. DKK4, like DKK1, DKK2, and DKK3, possesses an N terminal signal peptide and 2 conserved cysteine-rich domains, which are separated by a linker region and contain 10 cysteine residues each. The second cysteine region has a putative lipid-binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin-type proteases. Northern blot analysis detected no expression of DKK4, but RT-PCR analysis detected DKK4 expression in cerebellum, T cell, esophagus, and lung cDNA libraries. DKK4 blocks Xenopus Wnt8, Wnt3a, and Wnt2b, but not Dsh or Fz8, induction of a secondary axis in frog embryos, indicating that DKKs antagonize WNT function upstream of WNT receptors.Synonyms: Dickkopf-related protein 4, Dkk-4
- ID gène
- 27121
- NCBI Accession
- NP_055235
- UniProt
- Q9UBT3
- Pathways
- Signalisation WNT
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