MLPH
Reactivité: Humain
Hôte: Lapin
Polyclonal
FITC
Indications d'application
Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.5-2 μg/mL. Approx 80 kDa band observed in Mouse Melanocyte extracts. Please note that this product may require overnight incubation of primary and high proteinloads. Immunofluorescence: Successfully used at 0.5 μg/mL to stain mouse melanocytes, seepicture. See also publication Figueiredo AC et al. , J Biol Chem.2008 Aug22,283(34): 23209-16. Epub 2008 Jun 17. (PMID: 18559336). GST-pull down assay: see publication Figueiredo AC et al. , J Biol Chem.2008 Aug22,283(34): 23209-16. Epub 2008 Jun 17. (PMID: 18559336). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
Tris saline, pH ~7.3 with 0.02 % Sodium Azide and 0.5 % BSA
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
4 °C/-20 °C
Stockage commentaire
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Melanophilin is a member of the exophilin subfamily of Rab effector proteins. It forms a ternary complex with the small Ras-related GTPase Rab27A in its GTP-bound form and the motor protein myosin Va. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.Synonyms: Exophilin-3, Leaden protein, Ln, Mlph, Slac2a, Slp homolog lacking C2 domains a, Synaptotagmin-like protein 2a